吡那地尔对缺血心肌保护作用的实验研究

目的: 探讨吡那地尔对大鼠在长时间缺血心肌的保护效果。方法: 取大白鼠18只,随机分为空白组、对照组和实验组,每组6只。灌注液分别采用S.t Thomas高钾停跳液、S.t Thomas高钾停跳液+川穹嗪、S.t Thomas高钾停跳液+吡那地尔。取下心脏后即刻及间隔30 min、40 min和50 min分别注射灌注液1次。收集第2、第3、第4次灌注后的心肌过滤液,检查心肌酶谱肌酸磷酸激酶(CPK)、肌酸激酶同工酶(CKMB)和肌钙蛋白I(CTNI),电镜观察心肌超微结构。结果: 实验组自第3次灌注后CPK、CKMB、CTNI低于对照组和空白组(P<0.05~P<0.01),心肌超微结构明显好于对照及空白组。结论: 吡那地尔对长时间缺血心肌有明显的保护作用。

Experimental study on the protective effect of pinacidil on the chronic ischemic myocardium

Objective: This study was designed to characterize the ultrastructure of the coccoid Helicobacter pylori(H. pylori) forms in the tissues of carcinoma.Methods: Transmission electron microscopy(TEM) technique was used to examine the structure of coccoid H. pylori in 4 cases of gastric carcinoma and 2 cases of esophageal carcinoma.Results: It was found that the coccoid H. pylori were scattered in cancer nest and cancer interspace,some of which enterded cancer cells,macrophages and other cells.The coccoid H. pylori appeared to be similar to the L-forms of other bacteria with varying cell shape and size,different cytoplasmic electron densities and defects in cell wall.Two types of coccoid H. pylori could be distinguished in the tissues of carcinoma by TEM.Type A was smaller with higher electron density in the cytoplasma,and flagellation on the cell membrane,suggesting that it could be viable.However,the type B was larger with lower electron density in the cytoplasma,and no flagellation on the cell membrane,suggesting that it could be a degenerative form.Conclusions: The coccoid forms of H.pulori still have certain degree of viability,thus they may play an important role in the transmission of H. pylori,the relapse of H. pylori related disease after treatment and the development of the related carcinoma.