Regulation of differentiation, proliferation and drug-induced apoptosis in HT58 lymphoma cells |
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Authors: | Rudolf Mihalik Ferenc Uher István PetÁk Anna SebestyÉn László Kopper |
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Institution: | (1) 1st Institute Pathology and Experimental Cancer Research, Semmelweis University of Medicine, üllői út 26, 1085 Budapest, Hungary;(2) National Institute of Hematology and Immunology, Budapest, Hungary |
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Abstract: | Recently, it has been suggested, that differentiated cells are more resistant to the apoptotic effect of DNA damaging agents
possibly due to the decreased activity of “damage detecting/apoptosis triggering” mechanism. Previously, we have shown, that
PMA pretreatment reduced etoposide-(ETO) but enhanced staurosporine- (STA) -induced apoptosis in HT58 cells. Data presented
here show that the HT58 human, “mature” B-lymphoma cells exposed to PMA secrete more IgM into the supernatant indicating commitment
of cells to perform differentiated function. The sensitivity of HT58 cells to ETO- or STA-induced apoptosis is influenced
diversely with PMA pre- or posttreatment. Interestingly, the DNA damage (gamma radiation, bleomycin, ETO) or okadaic acic
(30 nM) reduced the PMA+STA] - induced apoptosis. |
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Keywords: | apoptosis lymphoma differentiation etoposide staurosporine PMA |
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