自噬蛋白Beclin1和凝血指标监测与肺癌进展的相关性

目的 探讨肺癌患者自噬蛋白Beclin1、PLT、血浆D-二聚体、纤维蛋白原(Fib)含量变化,并观察其变化的趋势与肺癌临床分期、疗效和预后的关系.方法 采用流式细胞仪和全自动血凝仪以及全自动血细胞分析仪对50例不同病理分期的非小细胞肺癌患者和35例健康体检者(正常对照组)进行定量Beclin1和D-二聚体、血浆Fib水平和PLT检测.结果 与正常对照组比较,肺癌患者Beclin1蛋白的表达水平明显下降(24.90±4.68)％比(92.27±4.52)％],Fib和D-二聚体水平明显升高,差异有统计学意义(5.504±0.772) g/L比(2.953±0.719)g/L、( 1443.8±1664.5) μg/L比(148.8±82.4) μg/L,P＜0.01].肺癌组患者PLT计数为(506.60±65.21)×109/L,正常对照组为(210.94±47.34)×109/L,两者比较差异有统计学意义(P＜0.01).与正常对照组比较,随着病理恶性度的增加,肺癌组患者PLT明显增高,同时血浆Fib水平亦增高,差异有统计学意义(P＜0.01).结论 外周血PLT、血浆Fib和D-二聚体含量及自噬蛋白Beclin1的变化与肺癌的恶性度有明显相关性,联合多参数检测综合分析可作为临床疗效评估及判断预后的有效指标.

Relevance of the expression of Bedine 1, coagulation index and lung cancer

Objective To discuss the change of autophagy protein Beclinel,platelet(PLT),plasma D-dimer (D-D)and fibrinogen(Fib) in patients with lung cancer,and to observe the relationship of its change trend and lung cancer clinical stages,effect and prognosis.Methods Beclinel,D-D,plasma Fib level and PLT were quantitatively detected at different pathological staging of 50 cases non-small cell lung cancer patients and 35 cases healthy people.Results The decline of the expression of Beclinel protein in lung cancer patients than normal control group (24.90 ± 4.68) ％ vs (92.27 ± 4.52 ) ％ ],and the Fib and D -dimer expression level changes significantly increased(5.504 ±0.772) g/L vs (2.953 ±0.719)g/L,(1443.8 ± 1664.5) μg/L vs(148.8 ±82.4) μg/L,P ＜0.01 ].PLT count in lung cancer patients was (506.60 ±65.21 ) x 109/L,showing a statistically significant difference from normal group(210.94 ±47.34) x 109/L](P ＜ 0.05 ).Compared with normal group,with pathological malignant degree inccreasing, patients' plasma PLT, Fib level increased significantly (P ＜ 0.01 ).Conclusion Peripheral blood plasma PLT,Fib and D-D levels and autophagy protein Beclinel are correlated to lung cancer malignant degree,which can be used as indexes of the clinical efficacy evaluation and prognosis.