| 用随机扩增多态DNA（RAPD）技术鉴定野山人参 |
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| 引用本文： | 丁建弥,万树文,梅其春,邵永平. 用随机扩增多态DNA（RAPD）技术鉴定野山人参[J]. 中成药, 2001, 23(1): 3-5 |
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| 作者姓名： | 丁建弥 万树文 梅其春 邵永平 |
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| 作者单位： | 1. 上海雷允上药业有限公司神象参茸分公司,上海,200002
2. 复旦大学生命科学学院,上海,200433 |
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| 摘 要： | 目的：探索用随机扩增多态DNA(RAPD)技术鉴别野山参和栽培人参的可能性。方法：从经过形态鉴定的野山人参和栽培人参药材中提取DNA模板，运用RAPD技术在电泳中产生条带。结果：用80个引物，其中一个引物产生野山人参可重复的特征条带。结论：结果证明应用RAPD技术能有效地区别野山人参和栽培人参。
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| 关 键 词： | RAPD 野山人参 栽培人参 |
| 文章编号： | 1001-1528(2001)01-0003-02 |
| 修稿时间： | 2000-09-30 |
RAPD Differentiation of wild Ginseng Species |
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| DING Jian mi ,WAN Shu wen ,MEI Qi chun ,SHAO Yong ping. RAPD Differentiation of wild Ginseng Species[J]. Chinese Traditional Patent Medicine, 2001, 23(1): 3-5 |
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| Authors: | DING Jian mi WAN Shu wen MEI Qi chun SHAO Yong ping |
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| Affiliation: | DING Jian mi 1,WAN Shu wen 1,MEI Qi chun 2,SHAO Yong ping 2 |
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| Abstract: | Objective: To explore the potentiality of random amplified polymorphic DNA (RAPD) technique for the differntiation of wild and cultivated Ginseng.Methods: DNA templated were extracted from wild and cultivated Ginseng of the crude drugs that were identified by morphology, and RAPD technique was applied to produce electrophoresis pattern. Results: Among the 80 primers screened, one was able to generate reproducible band, characteristic RAPD marker band which was unique to each of wild Ginseng samples was identified. Conclusion: The results demonstrated that RAPD marker technique could effectively authenicate wild and cultivated Ginseng samples. |
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| Keywords: | RAPD wild Ginseng cultivated Ginseng |
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