急性脑梗死MRS研究模型的建立及病理学评价

目的建立稳定、可行的脑局灶性梗死动物模型,并通过功能性磁共振成像和病理学方法对其进行评价。方法采用S-D大鼠25只,随机分5组。A组为假手术对照组,B、C、D、E组大鼠分别用自创改良线栓法栓塞大鼠一侧大脑中动脉后3、6、12、24 h进行磁共振成像和波谱分析,检查结束后各组取2~3只进行病理观察,另2只TTC染色后行梗死灶形态学观察。结果对照组(A组)MR I、1H MRS及病理检查均未见异常。实验组DW I成像病灶呈高信号,1H MRS示B、C、D、E各组均见病灶NAA峰下降,Lac峰在各组均见升高,B、C、D组Cho、Cr峰未见明显异常,E组Cho、Cr峰略下降。结论该方法制作急性脑梗死实验模型是稳定的、可重复的,并且适合MRS实验研究。

Establishment and Pathological Evaluation of an Acute Cerebral Infarction Model for Magnetic Resonance Spectroscopy Study

Objective To establish a stable and feasible animal model of acute cerebral infarction,and to evaluate it with functional magnetic resonance imaging and pathology.Methods Twenty-five S-D rats were randomly divided into five groups,and there were 5 rats in each group.Rats in group A were sham-operated for control study.Unilateral middle cerebral artery occlusion(MCAO) was performed with improved thread in group B,C,D,E and were enrolled for MRI and MRS study at 3,6,12,24 hours after MCAO.All rats were examined by 1-hydrogen magnetic resonance spectroscopy(~1H MRS).Two or three rats in each group were sacrificed for 2,3,5-triphenyltetrazolium chloride(TTC) staining of the brain and two rats for pathological examination after MRS.Results Rats in group A showed no change in brain on ~1 H MRS or pathological study.~1H MRS of the rat brains after right MCAO showed a decrease of N-acetyl-aspartate(NAA),an increase of Lactate(Lac) in all groups.There was no significant change of Choline(Cho) and Creatine(Cr) peaks onrats in group B,C,D.The peaks of Cho and Cr were slightly dropped in group E.Conclusion The acute regional cerebral ischemic model in rats made by our approach is stable and reproducible,and it is suitable for evaluation and study with functional magnetic resonancespectroscopy accurately and sensitively.