| 非促有丝分裂型人酸性成纤维细胞生长因子对细胞增殖活性的影响 |
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| 引用本文： | 郭钦丽,汪小凤,孟娟,郑青. 非促有丝分裂型人酸性成纤维细胞生长因子对细胞增殖活性的影响[J]. 广东医学, 2005, 26(6): 758-760 |
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| 作者姓名： | 郭钦丽 汪小凤 孟娟 郑青 |
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| 作者单位： | 暨南大学,药学院,广州,510630;暨南大学,药学院医药生物技术研究开发中心,广州,510630 |
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| 基金项目： | 国家863计划资助项目(编号:2001AA215131,2002AA2Z3318)，广东省自然科学基金资助项目(编号:010424) |
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| 摘 要： | 目的研究观察人酸性成纤维细胞生长因子(haFGF)和N端缺失27个氨基酸残基的非促分裂人酸性成纤维细胞生长因子(non-mitogenetichumanacidicfibroblastgrowthfactor,nm-haFGF)对鼻咽癌细胞及血管内皮细胞增殖活性的影响。方法用不同浓度(0.01～100ng/ml)的人酸性成纤维细胞生长因子(haFGF)及nm-haFGF分别刺激鼻咽癌细胞及血管内皮细胞,作用48h后采用MTT法测定haFGF和nm-haFGF对两种细胞的增殖活性。结果在各浓度(>0.01ng/ml)下,nm-haFGF组对鼻咽癌细胞(除浓度为50ng/ml外)和血管内皮细胞的促增殖作用都显著低于haFGF组。结论nm-haFGF对鼻咽癌细胞及血管内皮细胞的促分裂活性下降。
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| 关 键 词： | 人酸性成纤维细胞生长因子 MTT法 有丝分裂 |
| 修稿时间： | 2004-11-03 |
The mitogenic activity of the non- mitogenetic human acidic fibroblast growth factor on nasopharyngeal and angioendothelial cells |
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| Guo Qin li,Wang Xiaofeng,Meng Juan,et al.. The mitogenic activity of the non- mitogenetic human acidic fibroblast growth factor on nasopharyngeal and angioendothelial cells[J]. Guangdong Medical Journal, 2005, 26(6): 758-760 |
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| Authors: | Guo Qin li Wang Xiaofeng Meng Juan et al. |
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| Affiliation: | Guo Qin li,Wang Xiaofeng,Meng Juan,et al. Pharmacy College of Jinan University,Guangzhou 510630 |
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| Abstract: | Objective To study the mitogenesis of non-mitogenetic human acidic fibroblast growth factor (nm-haFGF) on nasopharyngeal and angioendothelial cells. Methods The mitogenic effects of human acidic fibroblast growth factor (haFGF) and nm-haFGF on CNE2 cells and ECV304 cells were measured by MTT method. Results At different concentration(>0.01 (ng/ml)), the mitogenic effects of nm-haFGF on nasopharyngeal cells(except for the concentration of 50 (ng/ml)) and angioendothelial cells was significantly lower than that of haFGF. Conclusion The mitogenic effect of nm-haFGF on CNE2 cells and ECV304cells is lower than that of haFGF. |
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| Keywords: | Human acidic fibroblast growth factor MTT Mitosis |
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