I mmunomodulatory effects of murine melanoma cells transduced with interleukin-2 gene and recombinant interferon-γ

The murine interleukin-2 gene was introduced intomurine melanoma B16 cells by retroviral vector. AfterG418 selection, a clone stably secreting high level ofinterleukin-2 (1038IU per 10~6 cells per 24 hours) wasobtained. The tumorigenicity of this clone decreasedsignificantly as compared with parental tumor cells andtumor cells transferred neomycin gene. There was notumor developed after injection of 5×10~5 IL2-transfectedB16 cells into C57BL/6 mice. However when the amount