通腑利肺作用机制的实验研究

目的 从整体、细胞、分子3个层面探讨通腑法通腑利肺的作用机制。方法 将40只SD大鼠随机等分为正常组、模型组、解扎组及治疗组，每组各10只，采用体外直肠不全结扎法造模，治疗组于术后48h从体外解除直肠结扎丝线并继续常规饲养，同时每天以大承气汤灌胃2次，每只每次2ml，服用2天。采用放射免疫分析法检测各组血清IL-8含量，RT-PCR法检测肺组织TNF-α mRNA水平，检测各组支气管肺泡灌洗液（BALF）中肺泡巨噬细胞（PAM）死亡百分率。结果模型组大鼠血清IL-8含量较正常组明显增加（P〈0．01），解扎组与治疗组IL-8含量较模型组明显降低（P〈0．05，P〈0．01）；治疗组较解扎组明显降低（P〈0．05）。解扎组、治疗组BALF中PAM总数较模型组升高，PAM死亡百分率较模型组降低（均P〈0．05）；治疗组BALF中PAM死亡百分率较解扎组降低（P〈0．05）。模型组TNF-α mRNA表达明显高于正常组，给予大承气汤治疗可降低肺组织TNF-α mRNA表达。结论 通腑法对损伤肺组织起保护作用。

Experimental Study on Acting Mechanism of Vicera Purging Method in Purging Fu-organs and Benefiting Fei

OBJECTIVE: To explore the acting mechanism of viscera purging method (VP) in purging Fu-organs and benefiting Fei from integral, cellular and molecular levels. METHODS: Forty SD rats were equally divided into four groups randomly: the normal group, the model group, the unhitch group and the VP group. Except those in the normal group were untreated, rats were established to intestinal obstruction model by incomplete ligation of the rectum in vitro. The ligation was relieved 48 h after operation in the unhitch group and the VP group, and the animals were fed continuously on routine. Meanwhile, Dachengqi Decoction (DD) 2 ml was given twice a day to the VP group for 2 days. Finally, the serum interleukin 8 (IL-8) and mRNA expression of tumor necrosis factor-alpha (TNF-alpha) in the lung tissue were detected by radioimmunoassay and RT-PCR respectively. Besides, the number of pulmonary alveolar macrophages (PAM) in the bronchial alveolus lavage fluid (BALF) was counted and their death rate calculated. RESULTS: Compared with the normal control, the serum IL-8 content in lung tissue in the model rats were remarkably higher (P < 0.01); however, the VP group showed the lowest level of IL-8 content and the highest was shown in the model group. Number of PAM in BALF was higher and its death rate was lower in the VP group than that in the unhitch groups (both P< 0.05). The expression of TNF-alpha mRNA was sinificantly higher as compared with that in the normal group, and lowered after administration of DD. CONCLUSION: Viscera purging method could protect the injured lung tissue to some extent.