存活蛋白和P63蛋白在B细胞非霍奇金淋巴瘤组织中的表达及其对细胞凋亡和增殖的作用

本研究探讨存活蛋白（Survivin）和P63蛋白在B细胞非霍奇金淋巴瘤（B—NHL）发生发展中的可能作用及其与细胞凋亡和增殖的相互关系。应用免疫组织化学SP法和TdT介导的dUTP缺口末端标记（TUNEL）技术检测43例B-NHL和10例反应性增殖淋巴组织（RHL）中的survivin和P63蛋白表达以及细胞凋亡和增殖细胞核抗原（PCNA）的水平。结果表明：survivin和P63在43例B-NHL中表达的阳性率分别为69．8％（30／43）和82．7％（36／43），survivin和P63在10例RHL中表达阳性率分别为10％（1／10）和40％（4／10），survivin在侵袭性B—NHL中的阳性率明显高于惰性B—NHL（83．3％vs46．2％，P〈0．01），P63蛋白表达差异无显著性意义；survivin阳性表达与凋亡指数（AI）和增殖指数（PI）呈正相关（r=0．429，P〈0．01；r=0．348，P〈0．01），P63蛋白阳性表达与AI和PI呈正相关（r=0．451，P〈0．01；r=0．369，P〈0．05），B—NHL的AI和PI呈显著正相关（r=0．598，P〈0．001）。结论：survivin可能参与了B—NHL的凋亡和增殖调控机制，P63作为癌基因参与B—NHL发生，二者可能共同影响细胞增殖和凋亡。

Expression of Survivin and P63 Protein in B Cell Non-Hodgkin''''s Lymphoma and Their Effects on Cell Apoptosis and Proliferation

The study was aimed to explore the possible roles of survivin and P63 protein in the development and progression of B cell non-Hodgkin's lymphoma(B-NHL)and their relation with cell apoptosis and proliferation.TdT-mediated dUTP nick end labeling(TUNEL)and immunohistochemistry were used to detect the survivin and P63 protein expression,cell apoptosis and proliferating cell nuclear antigen(PCNA)level in 43 cases of B-NHL and 10 cases of reactive hyperplasia lymphoid(RHL)tissues.The results indicated that the positive rates of survivin and P63 protein expression were 69.8%(30/43)and 82.7%(30/43)respectively.The expression of survivin and P63 protein was 10%(1/10)and 40%(4/10)in RHL tissues of 10 cases.The expression of survivin in aggression B-NHL was higher than that in indolent B-NHL(83.3% vs 46.2%,P<0.01).The expression of P63 proteins in aggressive B-NHL was higher than that in indolent B-NHL(86.7% vs 76.9%,P>0.05).Apoptotic index(AI)and proliferation index(PI)correlated positively with expression of survivin(r=0.429,P <0.01;r=0.348,P<0.01),and so do with expression of P63 proteins(r=0.451,P <0.01;r=0.369,P<0.05).In addition,AI and PI were positively related(r=0.598,P<0.001).It is concluded that survivin may participate in the regulation mechanism of B-NHL cell apoptosis and proliferation,P63 as an oncogene enhances proliferation and takes part in the development of B-NHL.There may be a close relationship between survivin and P63 protein in the regulation of lymphocyte proliferative kinetics.