| 柱前衍生化法测定葫芦巴中4-羟基异亮氨酸的含量 |
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| 引用本文： | 李波,曾光尧,谭健兵,周应军,谭桂山.柱前衍生化法测定葫芦巴中4-羟基异亮氨酸的含量[J].中南药学,2009,7(4):277-279. |
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| 作者姓名： | 李波 曾光尧 谭健兵 周应军 谭桂山 |
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| 作者单位： | 1. 湖南省药品食品监督管理局,长沙,410013
2. 中南大学药学院,长沙,420013 |
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| 摘 要： | 目的 建立葫芦巴中4-羟基异亮氨酸的含量测定方法。方法采用反相高效液相色谱、柱前邻苯二甲醛衍生化法。Kromasil ODS（250mm×4．6mm，5μm）色谱柱为固定相，甲醇-0．05mol·L。醋酸钠水溶液（pH=7．2，其中加入0．5％四氢呋喃）梯度洗脱（0min～20min，30：70；30min，40：60），检测波长为340nm，柱温为30c，流速为1mL·min^-1。结果4-羟基异亮氨酸在0．1848～3．696μg与峰面积呈良好的线性关系（r=0．999），平均回收率-99．12％．RSD=2．15％（n=6）。结论测定方法简便、准确，首次建立了葫芦巴药材的4-羟基异亮氨酸的邻苯二甲醛柱前衍生化含量测定方法，为葫芦巴质量控制及综合利用提供了依据。
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| 关 键 词： | 葫芦巴 4-羟基异亮氨酸 反相高效液相色谱法 柱前衍生化法 |
Content determination of 4-hydroxyisoleucine inTriganellaj oenumgraecum L.by pre-column derivatization of HPLC |
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| LI Bo,ZENG Guang-yao,TAN Jian-bing,ZHOU Ying-jun,TAN Gui-shan.Content determination of 4-hydroxyisoleucine inTriganellaj oenumgraecum L.by pre-column derivatization of HPLC[J].Central South Pharmacy,2009,7(4):277-279. |
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| Authors: | LI Bo ZENG Guang-yao TAN Jian-bing ZHOU Ying-jun TAN Gui-shan |
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| Institution: | 1. Hunan Food and Drug Ad- ministration, Changsha 4100131 2. School of Pharmaceutical Sciences, Central South University, Changsha 410013) |
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| Abstract: | Objective To establish a method for the content determination of 4-hydroxyisoleucine in Triganellaj oenumgraecum L. Methods A pre-column derivation was developed by HPLC with o-phthaladehyde. HPLC was used with Kromasil ODS（250 mm×4.6 mm, 5μm）column, methanol-0. 5mol·L^-1 sodium acetate（0 min-20 min, 30 ：70; 30 min, 40 ： 60） was used as the mobile phase. The detection wavelength was 340 nm and the flow rate was 1 mL·min^-1. Results The calibration curve of 4-hydroxyisoleucine was linear at 0. 184 8～3. 696μg （r=0. 999）. The average recovery was 99.12%, RSD=2.15% （n=6） . Conclusion The method is rapid, accurate and suitable for the quality control of Triganellaj oenumgraecum L. |
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| Keywords: | Triganellaj oenumgraecum L 4-hydroxyisoleucine RP-HPLC pre-eolumn derivation |
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