| 谷氨酸影响培养的海马神经元内钙变化机制 |
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| 引用本文： | 谭来勋,孙圣刚,管强.谷氨酸影响培养的海马神经元内钙变化机制[J].武汉大学学报(医学版),2006,27(6):707-710. |
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| 作者姓名： | 谭来勋 孙圣刚 管强 |
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| 作者单位： | 1. 武汉大学人民医院神经内科,武汉,4300602;华中科技大学同济医学院附属协和医院神经内科,武汉,430022
2. 华中科技大学同济医学院附属协和医院神经内科,武汉,430022 |
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| 基金项目： | 高等学校博士学科点专项科研项目 |
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| 摘 要： | 目的:研究谷氨酸对培养的大鼠海马神经元内钙信号的影响及作用机制。方法:用显微荧光测量技术监测神经元内钙信号的动态变化和谷氨酸对其的影响;阻断NMDA、AMPA受体后,观察谷氨酸对神经元内钙信号影响的变化。结果:谷氨酸使神经元内游离钙浓度明显升高,NMDA和AMPA受体拮抗剂、胞外钙离子浓度的降低均可不同程度地降低胞内游离钙离子升高幅度。结论:谷氨酸通过多种途径影响大鼠海马神经元内钙信号,激活NMDA和AMPA受体是其中的重要机制之一。
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| 关 键 词： | 神经元 谷氨酸 NMDA受体 AMPA受体 细胞内钙 |
| 文章编号： | 1671-8852(2006)06-0707-04 |
| 收稿时间： | 2006-01-14 |
| 修稿时间： | 2006年1月14日 |
Mechanism for Glutamate Induced Intracellular Calcium Change in Cultured Hippocampal Neurons of Rats |
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| TAN Laixun,SUN Shenggang,GUAN Qiang.Mechanism for Glutamate Induced Intracellular Calcium Change in Cultured Hippocampal Neurons of Rats[J].Medical Journal of Wuhan University,2006,27(6):707-710. |
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| Authors: | TAN Laixun SUN Shenggang GUAN Qiang |
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| Institution: | 1. Dept. of Neurology, Renmin Hospital of Wuhan University, Wuhan 430060, China ;2.Dept. of Neurology, Union Hospital, Tongji Medical College of Huazhong Science Technology, Wuhan 430022, China |
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| Abstract: | Objective: To study the effect of glutamate on the intracellular calcium signal of cultured rat hippocampal neurons and its mechanism.Methods: Using microfluorescent technique to detect the calcium signal and the effect of glutamate on it and observing its change upon the antagonization of NMDA and AMPA receptors simultaneously or respectively.Results: Glutamate could increase the intracellular free Ca~(2 )(Ca~(2 )]i) concentration obviously,and the antagonists of NMDA and AMPA receptors,low levels of extracellular calcium concentration and the calcium chelator can attenuated the response at different extent.Conclusion: It is suggested that glutamate affects intracellular calcium signal by complex mechanisms,and the activation of NMDA and AMPA receptors is an important one of them. |
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| Keywords: | Neuron Glutamate NMDA Receptor AMPA Receptor Intracellular Calcium |
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