| 高效液相色谱法测定姜连止泻片中盐酸小檗碱的含量 |
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| 引用本文： | 孟蕾,沈圣民,张海鸣,李宝林,杜树山.高效液相色谱法测定姜连止泻片中盐酸小檗碱的含量[J].时珍国医国药,2010,21(2). |
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| 作者姓名： | 孟蕾 沈圣民 张海鸣 李宝林 杜树山 |
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| 作者单位： | 1. 北京师范大学校医院,北京,100875
2. 北京师范大学资源学院教育部资源药物工程研究中心,北京,100875 |
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| 基金项目： | 北京市科委重大培育专项 |
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| 摘 要： | 目的 建立姜连止泻片中盐酸小檗碱的高效液相色谱(HPLC)含量测定方法.方法 采用HPLC法,色谱柱:Waters Symmetry Shield~(TM) RP_(18) 柱(150 mm×3.9 mm,5 μm),流动相:乙腈-0.05mol·L~(-1)磷酸二氢钾溶液(用磷酸调节pH值至2.0)(22:78),检测波长:270 nm,流速:1.0 ml·min~(-1),柱温:30℃.结果 盐酸小檗碱在0.12～0.59 μg范围内呈现良好的线性关系(r=0.999 7),加样回收率为98.30%,RSD为1.19%(n=6).结论 该方法快速、简便、准确、重复性较好,结果可靠,可用于控制姜连止泻片制剂的质量.
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| 关 键 词： | 姜连止泻片 盐酸小檗碱 高效液相色谱法 |
Determination of Berberine Hydrochloride in Jianglianzhixie Tablets by HPLC |
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| MENG Lei,SHEN Sheng-min,ZHANG Hai-ming,LI Bao-lin,DU Shu-shan.Determination of Berberine Hydrochloride in Jianglianzhixie Tablets by HPLC[J].Lishizhen Medicine and Materia Medica Research,2010,21(2). |
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| Authors: | MENG Lei SHEN Sheng-min ZHANG Hai-ming LI Bao-lin DU Shu-shan |
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| Institution: | MENG Lei1,SHEN Sheng-min2,ZHANG Hai-ming2,LI Bao-lin2,DU Shu-shan2 (1.School Hospital of Beijing Normal University,2.College of Resources Science & Technology,Center for Natural Medicine Engineering,China Ministry of Education,Beijing Normal University,Beijing 100875,China) |
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| Abstract: | Objective To establish an accurate method for the determination of berberine hydrochloride in Jianglianzhixie Tablets.Methods Waters Symmetry ShieldTM RP18 column (150 mm×3.9 mm,5 μm) in an oven at 30℃ was need,with a mobile phase consisting of acetonitrile-0.05mol·L~(-1) potassium dihydrogen phosphate(phosphate regulation pH value to 2.0) (22:78) and a UV detector at 270 nm, the flow rate was 1.0 ml·min~(-1). Results The linear range of berberine hydrochloride was within 0.12～0.59 μg. The average recovery was 98.30% and RSD was 1.19%(n=6).Conclusion The method is simple and accurate, and can be used for the quality control of Jianglianzhixie tablets. |
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| Keywords: | Jianglianzhixie Tablets Berberine hydrochloride HPLC |
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