高效液相色谱法测定酪胺含量 |
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引用本文: | 王威,任永辉,司春庄,宋立强. 高效液相色谱法测定酪胺含量[J]. 黑龙江医药, 2010, 23(3): 322-323 |
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作者姓名: | 王威 任永辉 司春庄 宋立强 |
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作者单位: | 牡丹江恒远药业有限公司,黑龙江牡丹江,157009;牡丹江恒远药业有限公司,黑龙江牡丹江,157009;牡丹江恒远药业有限公司,黑龙江牡丹江,157009;牡丹江恒远药业有限公司,黑龙江牡丹江,157009 |
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摘 要: | 目的:建立快速、准确测定酪胺含量的高效液相色谱方法。方法:采用安捷伦HPLC1200系统,色谱柱采用Agilent Eclipse XDB—C18填料(250mm×4.6mm,5um),流动相:0.02mol/L磷酸氢二钠-乙腈(85:15)用10%磷酸调节pH值为8.3,流速:1.0ml/min;在225nm波长处检测。结果:酪胺在8.0~160ug·ml-1 的浓度范围内呈线性关系,r=0.9999。
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关 键 词: | 高效液相色谱法 酪胺 含量测定 |
Determination of Tyramine Content by HPLC |
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Affiliation: | Wang Wei, et al (Mudanjiang Hengyuan Pharmaceutical Co.,Ltd (157000)) |
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Abstract: | Objective: To establish a rapid and accurate determination of tyramine content in high-performance liquid chromatography. Methods: Agilent HPLC 1200 system, column using Agilent Eclipse XDB-C18 filler (250mm × 4.6mm, 5um), mobile phase: 0.02mol / L disodium hydrogen phosphate - acetonitrile (85:15) with 10% phosphoric acid adjusted pH, 8.3, flow rate: 1.0ml/min; detection wavelength at 225nm.Results:Tyraminc in the 8.0 - 160 ug. ml-1 within the concentration range of linear relationship, r = 0.99990 |
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Keywords: | High-performance liquid chromatography Tyramine Content determination |
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