| HPV18 E6 siRNA对宫颈癌细胞增殖及化疗药物敏感性的影响 |
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| 引用本文： | 李晶华,王伟娟,高婉丽,郭蕾,冯力民. HPV18 E6 siRNA对宫颈癌细胞增殖及化疗药物敏感性的影响[J]. 基础医学与临床, 2010, 30(5): 500-504 |
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| 作者姓名： | 李晶华 王伟娟 高婉丽 郭蕾 冯力民 |
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| 作者单位： | 首都医科大学,附属北京天坛医院妇产科,北京,100050 |
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| 基金项目： | 2007年度首都医学发展科研基金(2007-3097) |
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| 摘 要： | 目的探讨HPV18 E6siRNA联合紫杉醇应用对宫颈癌细胞生长的影响。方法用RNAi抑制HeLa细胞中HPV18 E6蛋白的表达。Western blot检测E6、P53和P21的蛋白表达量,MTT法检测细胞的增殖活性及细胞对紫杉醇的敏感性。结果抑制HPV18 E6的表达后,P53和P21的表达量均显著升高,HeLa细胞的生长受到抑制(P<0.01)。加入紫杉醇后,细胞增殖速度较对照组明显降低(P<0.01)。结论HPV18 E6siRNA可有效沉默HeLa细胞中E6基因的表达,抑制细胞增殖,并提高细胞对化疗药物的敏感性。
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| 关 键 词： | 宫颈癌 HPV18 E6 siRNA 紫杉醇 细胞增殖活性 化疗敏感性 |
| 收稿时间： | 2009-12-21 |
| 修稿时间： | 2010-03-06 |
Effect of HPV E6 siRNA on the proliferation and chemotherapy sensitivity of cervical cancer HeLa cells |
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| LI Jing-hua,WANG Wei-juan,GAO Wan-li,GUO Lei,FENG Li-min. Effect of HPV E6 siRNA on the proliferation and chemotherapy sensitivity of cervical cancer HeLa cells[J]. Basic Medical Sciences and Clinics, 2010, 30(5): 500-504 |
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| Authors: | LI Jing-hua WANG Wei-juan GAO Wan-li GUO Lei FENG Li-min |
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| Affiliation: | Dept.of Gynecology and Obstetrics;Affiliated Beijing Tiantan Hospital of Capital Medical University;Beijing 100050;China |
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| Abstract: | Objective To elucidates the effects of HPV18 E6 siRNA targeting at human papillomavirus(HPV) 18 E6 gene on the proliferative activity of HeLa cells and chemotherapy sensitivity.Methods HPV18 E6 expression of HeLa cells was inhibited by siRNA interference,the change of P53 and P21 proteins expression level was measured by Western blot.MTT assay was used to detected proliferative activity and sensitivity to paclitaxel liposome of HeLa cells.Results After inhibition of E6 expression,P53 and P21 proteins increa... |
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| Keywords: | cervical cancer HPV18 E6 siRNA paclitaxel liposome cell proliferation activity chemotherapy sensitivity |
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