| 化疗药上调DR4、DR5表达体外逆转人骨肉瘤对TRAIL的耐受 |
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| 引用本文： | 杨彩虹,陈安民,曾恒,陈剑锋.化疗药上调DR4、DR5表达体外逆转人骨肉瘤对TRAIL的耐受[J].临床外科杂志,2008,16(10):690-691. |
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| 作者姓名： | 杨彩虹 陈安民 曾恒 陈剑锋 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院骨科,武汉,430030 |
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| 摘 要： | 目的研究肿瘤坏死因子相关凋亡诱导配体（TRAIL）与化疗药对人骨肉瘤的联合作用及其受体（TRAILR）在人骨肉瘤中的表达间的关系。方法应用细胞计数、AO／EB染色、流式细胞术等方法比较检测阿霉素、顺铂、甲氨喋呤、紫杉醇单独作用和与TRAIL联合作用于MG-63及骨肉瘤组织的细胞毒效应；应用原位杂交、Western Blot方法检测人骨肉瘤细胞系MG-63及新鲜骨肉瘤组织用药前后TRAILR的表达。结果阿霉素、顺铂和紫杉醇增强死亡受体的表达，且与TRAIL联合作用诱导人骨肉瘤细胞凋亡的效率显著强于单用。结论阿霉素、顺铂、紫杉醇通过上调DR4、DR5表达逆转人骨肉瘤对TRAIL的耐受。
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| 关 键 词： | 骨肉瘤 肿瘤坏死因子相关凋亡诱导配体 阿霉素 紫杉醇 顺铂 |
Chemotherapeutic agents sensitize osteosarcoma cells to TRAIL-induced apoptosis by inducing death receptors 4 and 5 protein levels in vitro |
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| YANG Cai-hong,CHEN An-min,ZENG Heng,et al..Chemotherapeutic agents sensitize osteosarcoma cells to TRAIL-induced apoptosis by inducing death receptors 4 and 5 protein levels in vitro[J].Journal of Clinical Surgery,2008,16(10):690-691. |
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| Authors: | YANG Cai-hong CHEN An-min ZENG Heng |
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| Institution: | YANG Cai-hong,CHEN An-min,ZENG Heng,et al.(Department of Orthopedics,Tongji Hospital,Tongji Medical College,Huazhong University of Science , Technology,Wuhan 430030,China) |
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| Abstract: | Objective To study the relationship between the effect of tumor related apoptosis inducing-ligand(TRAIL)on human osteosarcoma cells after which were pretreated with chemotherapeutic agents and the expression of TRAIL receptors in the cells.Methods The expression of TRAIL receptors in human osteosarcoma cell line MG-63 and fresh osteosarcoma specimens was detected by in situ hybridation and western blot before and after the cells and tissue specimens were treated with TRAIL;MTT and flow cytometry(FCM)were us... |
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| Keywords: | osteosarcoma tumor necrosis factor-related apoptosis inducing-ligand adriamycin paclitaxel cisplatin |
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