Expanding the forensic German mitochondrial DNA control region database: genetic diversity as a function of sample size and microgeography |
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Authors: | H Pfeiffer B Brinkmann J Hühne B Rolf A A Morris R Steighner M M Holland P Forster |
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Institution: | Institute of Legal Medicine, University of Münster, Von-Esmarch-Stra?e 62, D-48149 Münster, Germany Fax: +49-251-83-55-158, DE Earswick Drive, Scarborough, Ontario, M1 E 1C6, Canada, CA Armed Forces DNA Identification Laboratory, Armed Forces Institute of Pathology, 1413 Research Blvd., Rockville, MD, USA 20850D3125, US
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Abstract: | Mitochondrial DNA control region sequences were determined in 109 unrelated German Caucasoid individuals from north west
Germany for both hypervariable regions 1 (HV1) and 2 (HV2) and 100 polymorphic nucleotide positions (nps) were found, 63 in
HV1 and 37 in HV2. A total of 100 different mtDNA lineages was revealed, of which 7 were shared by 2 individuals and 1 by
3 individuals. The probability of drawing a HV1 sequence match within the north west Germans or within published sets of south
Germans and west Austrians is similar (within a factor of 2) to drawing a sequence match between any two of these three population
samples. Furthermore, HV1 sequences of 700 male inhabitants of one village in Lower Saxony were generated and these showed
a nearly linear increase of the number of different haplotypes with increasing number of individuals, demonstrating that the
commonly used haplotype diversity measure (Nei 1987) for population samples tends to underestimate mtDNA diversity in the
actual population.
Received: 14 January 1999 / Accepted: 15 February 1999 |
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Keywords: | Mitochondrial DNA Hypervariable regions Population study Match probability |
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