三叶因子2在慢性支气管哮喘大鼠肺组织中的表达

目的：进一步探讨三叶因子2(TFF2)在慢性支气管哮喘(简称哮喘)大鼠肺组织中的表达。方法24只雄性 Wistar 大鼠随机分为3组(每组8只)：①正常大鼠组(A 组)：无特殊处理。②鸡卵白蛋白(ovalbumin,OVA)致敏生理盐水吸入组(B 组)：应用 OVA 致敏大鼠,生理盐水激发；③慢性哮喘组(C 组)：应用 OVA 致敏和反复激发的方法制备大鼠慢性哮喘模型。各组大鼠于末次激发后24 h 处死。ELISA 法测定 BALF 中 TFF2水平；肺组织标本行 HE 染色、PAS 染色及Masson 三色染色,并行病理图像形态学测定和分析；免疫组织化学检测大鼠气道上皮细胞 TFF2的表达。结果 C 组大鼠气道上皮细胞 TFF2的表达与 A 组及 B 组相比明显增多(A 组与 C 组比较 q =74.51,P <0.01,B 组与 C 组比较 q =71.93,P <0.01)。C 组大鼠 BALF 中 TFF2水平与 A 组及 B 组相比明显增高(A 组与 C 组比较 q =7.16,P <0.01,B 组与 C 组比较 q =6.13,P <0.01)。结论TFF2在慢性哮喘大鼠肺组织中表达增多。

Expressions of trefoil factor 2 in lung tissue of chronic asthma rats

Objective To investigate the expression of trefoil factor 2 (TFF2) in lung tissue of chronic bronchial asthma(asthma) rats.Methods Twenty four male Wistar rats were randomly divided into 3 groups.Rats in group A were given no special treatment.Rats in group B were sensitized with ovalbumin(OVA) and challenged with saline.Rats in group C were sensitized and challenged with OVA to set up chronic asthma model.The expression of TFF2 in BALF were detected using ELISA.Lung sections were stained with hematoxylin and eosin (HE),periodic acid Schiff (PAS),and Masson trichrome,and the images were analyzed morphometrically.The expression of TFF2 in the airways were detected using immunohistochemistry.Results The expression of TFF2 in the airways of rats in group C was upregulated than that in group A and group B(q =74.5 1,P <0.01,q =71.93,P <0.01),and the TFF2 level in BALF in the rats of group C was higher than that in group A and group B(q =7.1 6,P <0.01,q =6.13,P <0.01).Conclusions The expression of TFF2 were upregulated in the lung tissue of chronic asthma rats.