汉防己甲素联合顺铂对 NCI-H446细胞的影响及相关机制分析

目的：研究汉防己甲素(tetrandrine,TET)联合顺铂(cisplatin,DDP)对人小细胞肺癌细胞株 NCI-H446体外抗肿瘤效果,探讨 TET 增强 NCI-H446细胞对 DDP 敏感性的可能机制。方法体外培养人小细胞肺癌株 NCI-H446；药物作用48 h 后,采用 CCK-8法测定各组细胞活力；Edu 染色观察各组细胞增殖情况；Hoechst 染色检测各组细胞凋亡情况；Western blot 检测各组磷酸化Akt、Bax、Bcl-2、cleaved-caspase-3、LC3等蛋白表达水平变化。结果低浓度的 TET 与 DDP 联合后可有效抑制 NCI-H446细胞株的增殖,诱导其凋亡；联合组半数抑制浓度较单药组显著降低；联合组磷酸化 Akt 表达显著下降,Bax、cleaved-caspase-3表达显著提高,抗凋亡蛋白 Bcl-2表达下降；自噬相关蛋白 LC3表达明显上调。结论低浓度 TET 与顺铂具有协同效应,其机制可能是通过上调促凋亡蛋白 Bax,下调抗凋亡蛋白 Bcl-2来诱导细胞凋亡；并且自噬可能在其中发挥重要作用。

Effects and potential mechanisms of tetrandrine combined with cisplatin in NCI-H446 cell line

Objective To investigate the anti-tumor activity of tetrandrine (TET) combined with cisplatin(DDP) in human small cell lung cancer cell line NCI-H446,to study the potential mechanism of TET enhancing the sensitivities of DDP on NCI-H446.Methods Human small cell lung cancer cell line NCI-H446 was routinely cultured in vitro.Cell viability of each group was analyzed with CCK-8 assay after treated with different drugs for 48 hours.The proliferation rate was determined by Edu staining. Hoechst staining was used to evaluate the apoptosis cells of NCI-H446.The expressions of the related proteins such as P-Akt,Bax,Bcl-2,cleaved-caspase-3,LC3 were detected by Western blot.Results The low concentration of TET combined with DDP exerted a synergistic effect on inhibiting proliferation and inducing apoptosis of NCI-H446 cell line.The IC50 of combination group was significantly lower than that of other groups.The phosphorylation level of Akt was decreased,and the levels of Bax and cleaved-caspase-3 were increased,the expression of anti-apoptosis protein Bcl-2 was decreased in the combination group.The autophagy related protein LC3 was up-regulated simultaneously.Conclusions The low concentration of TET has synergistic effect with DDP via inducing apoptosis of NCI-H446 by up-regulating pro-apoptosis protein Bax and down-regulating anti-apoptosis protein Bcl-2,and autophagy may play an important role in the apoptosis.