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| 细胞因子对人类胚胎干细胞分离克隆的影响 | |
| 引用本文： | 李松,窦忠英,宋文刚,华进联,张显忠,张彬彬. 细胞因子对人类胚胎干细胞分离克隆的影响[J]. 泰山医学院学报, 2003, 24(2): 91-92 |
| 作者姓名： | 李松 窦忠英 宋文刚 华进联 张显忠 张彬彬 |
| 作者单位： | 1. 泰山医学院免疫学教研室,山东,泰安,271000；陕西省干细胞研究中心,陕西,杨凌,712100 2. 陕西省干细胞研究中心,陕西,杨凌,712100 3. 泰山医学院免疫学教研室,山东,泰安,271000 |
| 基金项目： | 国家自然科学基金 (39970 36 3)，国家 973项目资助(G19990 5 430 0 ) |
| 摘 要： | 目的 观察白血病抑制因子、干细胞因子、碱性成纤维细胞生长因子对人类胚胎干细胞分离克隆的影响。方法在基础细胞培养液中加入不同的细胞因子组合，培养后观察人类胚胎干细胞集落形成数和各组的最高传代数。结果 细胞因子添加与否对人类胚胎干细胞原代培养无显著的影响，而对后继培养有显著影响。结论 在人类胚胎干细胞初代培养时可以不添加细胞因子，细胞的克隆过程中添加细胞因子有利于细胞的生长。 |
| 关 键 词： | 细胞因子 人类胚胎干细胞 分离克隆 |
| 文章编号： | 1004-7115(2003)02-0091-02 |
| 修稿时间： | 2002-12-09 |
Effects of cytokines on isolation and cloning of human embryonic stem-like cells |
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| LI Song,DOU Zhong-ying,SONG Wen-gang,HUA Jin-lian,ZHANG Xian-zhong,ZHANG Bin-bin. Effects of cytokines on isolation and cloning of human embryonic stem-like cells[J]. Journal of Taishan Medical College, 2003, 24(2): 91-92 | |
| Authors: | LI Song DOU Zhong-ying SONG Wen-gang HUA Jin-lian ZHANG Xian-zhong ZHANG Bin-bin |
| Abstract: | Objective: To investigate the effects of the cytokines on isolation and cloning of human embryonic stem-like cells.Methods: The different combination of cytokines were mixed with the culture medium of cells .After a period, the cloning number of human embryonic stem-like cells and the topmost passage were observed and registered. Results: There was not obvious effect on the primary culture of human ESC but the effect was distinct on the later passage if cytokines were used. Conclusion: In the primary passage , human ESC may survive without cytokines, and the impact of the existence of cytokines is better on the cloning of human ESC than that of its nonexistence. |
| Keywords: | cytokines human embryonic stem-like cells isolation and cloning |
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