IL-17、MMP-9在汉族和维吾尔族非小细胞肺癌患者中的表达及临床意义

目的检测白细胞介素-17（IL-17）和基质金属蛋白酶-9（MMP-9）在汉族、维吾尔族NSCLC患者中的表达，分析其表达在两民族中是否有差异，探讨其临床意义。方法随诊资料完整的术后NSCLC患者组织标本60例，其中汉族、维吾尔族各30例，采用PV9000二步免疫组织化学法检测IL-17和MMP-9在癌组织及癌旁组织中的表达。 结果IL-17、MMP-9在NSCLC中表达的阳性率为78.3%、80.0%，在癌旁组织中分别为48.3%、35.0%，癌组织中IL-17与MMP-9的表达高于癌旁组织(P<0.05)；IL-17的表达与肿瘤的肿块大小、淋巴结转移和TNM分期呈正相关(P<0.05),MMP-9与肿瘤大小、TNM分期呈正相关，差异有统计学意义(P<0.05)；IL-17和MMP-9的表达与患者的民族、年龄、性别、病理类型及分化程度差异无统计学意义(P>0.05)；汉族与维吾尔族的术后生存差异无统计学意义(P<0.05)，IL-7和MMP-9的阴性表达组的3年生存率较阳性表达组高，差异有统计学意义(P<0.05)。结论IL-17和MMP-9在癌组织中的表达不是汉族与维吾尔族患者在本院构成比差异的主要原因；IL-17和MMP-9的高表达对NSCLC的侵袭和转移起着重要作用；IL-17和MMP-9的高表达与肺癌的不良预后有关。

Expression and Clinical Significance of IL-17 and MMP-9 in NSCLC Patients of Han and Uighur

Objective To investigate the expression of IL-17 and MMP-9 in non-small cell cancer of the Han and Uighur NSCLC patients,analyze the differentiation of expression and the relationship between them and the clinical significance.Methods The expresion of IL-17 and MMP-9 were detected in 30 cases in Han and 30 cases in Uighur and lung tissues as control by PV9000 immunohistochemical technique.Results The positive expression of IL-17 and MMP-9 was detected in 60 cases NSCLC was 78.3% and 80.0%,respectively.Which was significantly higher than that in adjacent non-tumor tissues(P<0.05).The positive expression of IL-17 was related with the size of the tumor,TNM staging and also related to lymph node metastasis(P<0.05).The positive expression of MMP-9 was related with the size of tumor and TNM staging,but there was not correlated to nationality,age,gender,pathologic types and differentiation.The survival rate between the Han and Uighur is no sense in this study.The survival rate was different between NSCLC tissues with positive and negative IL-17 and MMP-9 expression(P<0.05).Conclusion The higher expression of IL-17 and MMP-9 in NSCLC is not the main factor about the different ratio of Han and Uighur in our hospital;IL-17 and MMP-9 may play important roles in regulating NSCLC invasion and metastasis.Over expression of IL-17 and MMP-9 could act as a reference to evaluate unfavorable prognosis of NSCLC.