消癌平注射液增敏奥沙利铂抑制卵巢癌Caov-3细胞的增殖

目的研究消癌平是否增敏奥沙利铂（Oxaliplatin，OXA）抑制卵巢癌细胞的增殖，并探讨其药理机制。方法体外培养的卵巢癌Caov-3细胞，分别施以OXA、OXA＋消癌平干预，空白对照组给予PBS，采用MTT法测定OXA 单独应用或与消癌平联合应用的IC50及肿瘤细胞存活率；荧光显微镜观察Caov-3细胞胞核形态变化；流式细胞术检测细胞凋亡；蛋白质免疫印迹方法检测caspase-3蛋白质表达。结果消癌平可明显降低OXA的IC50，抑制Caov-3细胞增殖；消癌平增敏OXA诱导的Caov-3细胞凋亡，增加细胞凋亡率，并促进caspase-3活化。结论消癌平增敏OXA抑制卵巢癌细胞Caov-3增殖，促进caspase-3活化可能是其增敏的机制之一。

Effect of Xiaoaiping Injection Sensitized Oxaliplatin on Inhibition of Human Ovarian Cancer Caov-3 Cells Proliferation

Objective
To investigate the sensitiziation of xiaoaiping injection to oxaliplatin in inhibitory of human ovarian cancer Caov-3 cells proliferation and explore the mechanism.MethodsCaov-3 cells were treated with oxaliplatin combined with or without xiaoaiping,and the control group was treated with PBS.The inhibitory effects on cell proliferation were examined by MTT assay and oxaliplatin’s IC50 was generated.The apoptosis morphological changes in Caov-3 cells were observed by fluorescent microscopy.Apoptosis peak was analyzed with FACScan flow cytometry.The expression of caspase-3 in Caov-3 cells was analyzed by Western blot.ResultsIn Caov-3 cells,the value of IC50 of OXA combined with xiaoaiping were significantly lower than that of OXA alone.And cell survival rate of the group of OXA combined with xiaoaiping was lower than that of group treated by OXA alone,too.Besides,xiaoaiping increased OXA induced apoptosis in morphological and DNA contents’ changes.In addition,the cleavage of caspase-3,a hallmark of apoptosis protein was increased in Caov-3 cells treated with oxaliplatin combined with xiaoaiping.ConclusionXiaoaiping can sensitize the OXA in inhibition of human ovarian cancer Caov-3 cells proliferation.Activation of caspase-3 might be involved in the mechanisms of the sensitization.