| RNA干涉法观察成纤维细胞生长因子18对成牙本质样细胞涎磷蛋白表达的影响 |
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| 引用本文： | 姜永,肖明振,杨帆,何文喜,范晓敏. RNA干涉法观察成纤维细胞生长因子18对成牙本质样细胞涎磷蛋白表达的影响[J]. 牙体牙髓牙周病学杂志, 2006, 16(5): 243-245 |
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| 作者姓名： | 姜永 肖明振 杨帆 何文喜 范晓敏 |
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| 作者单位： | 第四军医大学口腔医学院,陕西,西安,710032 |
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| 摘 要： | 目的：通过RNA干涉的方法研究成纤维细胞生长因子18（Fibroblast Growth Factor18．FGF18）对成牙本质样细胞中牙本质涎磷蛋白（dentin sialophosphoprotein，DSPP）表达的影响。方法：RNA干涉质粒pH1-siFGF18瞬时转染成牙本质样细胞MDPC-23，RT-PCR检测FGF18和DSPP的表达，观察FGF18低表达对DSPP表达的影响。结果：瞬时转染RNA干涉质粒后，MDPC-23细胞内FGF18表达明显减少，与此同时DSPP的表达量也明显减少。结论：FGF18可以调控DSPP的表达，影响成牙本质细胞的分化。
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| 关 键 词： | 成纤维细胞生长因子18 牙本质涎磷蛋白 RNA干涉 瞬时转染 |
| 文章编号： | 1005-2593（2006）05-0243-03 |
| 收稿时间： | 2005-09-27 |
| 修稿时间： | 2005-09-27 |
Investigation of the effect of FGF18 on DSPP expression of MDPC-23 by RNA interference |
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| JIANG Yong,XIAO Ming-zhen,YANG Fan,HE Wen-xi,Fan Xiao-min. Investigation of the effect of FGF18 on DSPP expression of MDPC-23 by RNA interference[J]. Chinese Journal of Conservative Dentistry, 2006, 16(5): 243-245 |
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| Authors: | JIANG Yong XIAO Ming-zhen YANG Fan HE Wen-xi Fan Xiao-min |
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| Abstract: | AIM:To investigate the effect of FGF18 on DSPP expression of MDPC-23 by means of RNA interference.METHODS: MDPC-23 cells were transiently transfected with RNA interference plasmid pH1/siFGF18, then the expression of FGF18 and DSPP were examined by RT-PCR. RESULT: With the decrease of FGF18 expression by RNA interference, the DSPP expression also decreased. CONCLUSION: FGF18 may modulate the expression of DSPP and participate in the development and differentiation of odontoblast. |
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| Keywords: | MDPC-23 |
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