Induction of matrix metalloproteinase-9 in mice during Toxocara canis larvae migration |
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Authors: | S C Lai K M Chen H C Chen H H Lee |
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Institution: | (1) Department of Parasitology, Chung Shan Medical University, 110, Section 1, Chien-Kuo North Road, 402 Taichung , Taiwan |
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Abstract: | The relationships between inflammation in organs with Toxocara canis larval migration and matrix metalloproteinase-9 (MMP-9) were investigated following the infection of mice with 1,000 infective eggs. Gelatinase activity was defined by gelatin zymography, optimum pH, inhibitor specificity and Western blot analysis. MMP-9 activity was present in the lungs, liver, muscles, and brain during T. canis larval migration. This enzyme had a molecular weight of about 94 kDa and showed maximum activity in the pH range of 6–8. The increased MMP-9 proteinases coincided with larval recovery and the degree of inflammation among the four organs. These results suggest that MMP-9 may be associated with the inflammatory reaction to larval toxocariasis during early migration, and may therefore be a useful marker during T. canis larvae migration. |
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