Cox-2和MMP-9在骨肉瘤组织中的表达及其临床意义

目的 观察Cox-2和MMP-9在骨肉瘤组织中的表达,探讨其在骨肉瘤发生、发展中的作用.方法 应用免疫组织化学SP法,检测35例骨肉瘤、14例骨软骨瘤中Cox-2和MMP-9的表达情况,并分析其在骨肉瘤不同病理分级、临床分期中的表达及两者之间的相互关系.结果 Cox-2蛋白在骨肉瘤中阳性表达率为82.9％(29/35),而在骨软骨瘤中阳性表达率为14.3％(2/14),两者比较差异有统计学意义(x2=20.232,P=0.000):MMP-9蛋白在骨肉瘤中阳性表达率为71.4％(25/35);而在骨软骨瘤中阳性表达率为35.7％(5/14),两者比较差异有统计学意义(x2 =5.373,P=0.020).Cox-2和MMP-9在骨肉瘤组织中的表达与Enneking分期、肺转移密切相关,且临床期别越高,二者的表达阳性率越高,且Cox-2蛋白与MMP-9蛋白的表达呈正相关(r=0.384.P=0.023).结论 Cox-2和MMP-9在骨肉瘤组织中均为高表达,提示它们可能对骨肉瘤的发生、发展起重要作用.

Expression of Cox-2, MMP-9 in Osteosarcoma and Their Clinical Significance

Objective To investigate the role of Cox-2 and MMP-9 in osteosarcoma by analyze their expression. Methotis The expression of Cox-2 and MMP-9 specimens of 35 osteosarcoma and 14 osteochondroma tissues was evalua- ted by immunohistochemistry. To analyze their expression and relationship in osteosarcoma different pathological grade, clinical stage. Results The positive rat（82, 90~ ~ 71.4~） of Cox-2 and MMP-9 in osteosarcoma were signifi- cantly higher than those in osteochondroma tissues（P〈0.01）. The expressions of Cox-2 and MMP-9 in osteosarco- ma were positively correlated（P〈0.05）. The expression of Cox-2 and MMP-9 were correlated with Enneking Staging and Lung metastases（P〈0. 05）. Conclusions Cox-2 and MMP-9 were highly expressed in osteosarcoma. It seems that Cox-2 and MMP-9 contribute to development of osteosarcoma.