氧化型低密度脂蛋白对培养的内皮细胞的致凋亡作用及尼莫地平的保护作用

通过研究氧化型低密度脂蛋白对培养的内皮细胞生长状态的影响及其致凋亡作用，阐述其致动脉粥样硬化机理，并研究尼莫地平对氧化型低密度脂蛋白诱导的内皮细胞凋亡的作用。用Cu^2 引发脂质过氧化过程，制备氧化型低密度脂蛋白，用MTT检测法检测细胞活性，PI杂色法及ELISA法检测细胞凋亡。结果显示，细胞生长对氧化型低密度脂蛋白呈浓度依赖性抑制作用。ELISA法和PI染色法进一步证实氧化型低密度脂蛋白可诱导内皮细胞发生凋亡。而尼莫地平可以抑制氧化型低密度脂蛋白诱导的内皮细胞凋亡。提示氧化型低密度脂蛋白对内皮细胞的细胞毒作用及其作用剂量有关；体内氧化型低密度脂蛋白通过致内皮细胞凋亡的途径，损伤血管内皮，引发动脉粥样硬化。尼莫地平可以对氧化型低密度脂蛋白诱导的内皮细胞凋亡起到保护作用。

Proatherogenic Effect of Oxidized Low Density Lipoprotein on Cultured Endothelium Cells and Protect Effect of Nimodipine

Aim To elucidate the proatherogenic effect of oxidized low density lipoprotein (ox-LDL)on the apoptosis of cultured endothelium cells and protect effect of nimodipine. Methods ox-LDL was obtained by incubated with Cu 2+ ,different concentrations of ox-LDL incubated with endothelium cells. MTT test was carried out to evaluate the cell growing state. PI staining test and ELISA test were conducted to analyze apoptosis index. Results The inhibiting effect of ox-LDL on the endothelium growth by ox-LDL was in a dose- dependent manner. ELISA and PI staining showed that ox-LDL induced endothelium cell apoptosis at the same concentration (P<0.05), Nimodipine could inhibited the ox-LDL induced endothelium cell apoptosis. Conclusions The cytotoxicity of ox-LDL to endothelium cell was related to the dosage concentration of ox-LDL: the ox-LDL could inhibit the cultured endothelial cells growth,and even resulted in apoptosis. ox-LDL could start the atherosclerosis in vivo due to the endothelium cells impairment and apoptosis.Nimodipine could protect endothelium from ox-LDL induced apoptosis.