| 大黄素对内毒素激活后巨噬细胞释放HMGB1的影响 |
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| 引用本文： | 王春新,徐明,陈国千.大黄素对内毒素激活后巨噬细胞释放HMGB1的影响[J].苏州大学学报(自然科学版),2010,30(1):87-89. |
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| 作者姓名： | 王春新 徐明 陈国千 |
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| 作者单位： | 无锡市人民医院检验科;卫生部寄生虫病预防与控制技术重点实验室; |
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| 基金项目： | 江苏省自然科学基金资助项目(BK2006027); 江苏省中医药局科研项目(H05178) |
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| 摘 要： | 目的探讨大黄素对内毒素激活后巨噬细胞高迁移率族蛋白B1（HMGB1）基因表达和胞外释放的影响。方法内毒素脂多糖激活后小鼠腹腔巨噬细胞培养物经不同浓度（5、20、50μmol/L）大黄素作用一定时间,观察培养上清液HMGB1、TNF-α含量的变化及细胞HMGB1mRNA表达的改变。结果大黄素（50μmol/L）明显抑制脂多糖激活后小鼠腹腔巨噬细胞HMGB1mRNA的表达和HMGB1、TNF-α的释放（均P（0.01）。结论大黄素具有抑制巨噬细胞HMGB1mRNA表达和HMGB1胞外释放的作用。
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| 关 键 词： | 大黄素 高迁移率族蛋白B1 肿瘤坏死因子 巨噬细胞 |
Effect of Emodin on HMGB1 Release from Endotoxin-stimulated Macrophages |
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| WANG Chun-xin,XU Ming,CHEN Guo-qian.Effect of Emodin on HMGB1 Release from Endotoxin-stimulated Macrophages[J].Suzhou University Journal of Medical Science,2010,30(1):87-89. |
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| Authors: | WANG Chun-xin XU Ming CHEN Guo-qian |
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| Institution: | WANG Chun-xin1,XU Ming2,CHEN Guo-qian1(1.Dept of Medical Laboratory,Wuxi People\'s Hospital,Jiangsu Wuxi 214023,China,2.Laboratory on Technology for Parasitic Disease Prevention , Control,Ministry of Health,Jiangsu Wuxi 214064,China) |
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| Abstract: | Objective To study the effect of emodin on mRNA expression and extracellular release of high mobility group box 1（HMGB1）in endotoxin-stimulated macrophages.Methods The changes of HMGB1 and TNF-α levels in the culture medium and expression of HMGB1 mRNA were investigated after Emodin（5、20、50 μmol/L）was incubated with lipopolysaccharide-stimulated cultured mouse peritoneal macrophages.Results Emodin（50 μmol/L）markedly suppressed the release of HMGB1 and TNF-α,and expression of HMGB1 mRNA from lipopolysaccharide-stimulated macrophages（P﹤0.01）.Conclusion Emodin confers inhibition onto extracellular release of HMGB1 and expression of HMGB1 mRNA from endotoxin-stimulated macrophages. |
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| Keywords: | emodin HMGB1 protein tumor necrosis factor-alpha macrophages |
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