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| 生血康口服液黄芪甲苷含量测定方法学研究 | |
| 引用本文： | 张杰,张凌艳,李贺一.生血康口服液黄芪甲苷含量测定方法学研究[J].黑龙江医药,2011,24(1):23-24. |
| 作者姓名： | 张杰 张凌艳 李贺一 |
| 作者单位： | 1. 哈药集团三精制药股份有限公司 2. 三精艾富西药业有限公司 3. 三精英美药业有限公司 |
| 摘 要： | 目的:建立高效液相色谱法测定生血康口服液的含量.方法:采用迪马(钻石)C<,18>(250mm×4.6mm.5μm)色谱柱,乙腈-水(35-65),流速1.0ml/min,蒸发光散射检测器.结果:黄芪甲苷与其它杂质能达到有效分离.在0.03021mg/ml～0.19206mg/ml范围内,浓度同峰面积呈良好的线形关系,... |
| 关 键 词： | 高效液相色谱法 黄芪甲苷 含量测定 |
Determination of Potassium Dehydroandrograpolide Succinate Injection by HPLC |
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| Institution: | Zhang Jie,et al Sanjing Pharmaceutical Co.Ltd.,Harbin Pharm.Group Harbin Sanjing FAC Pharmaceutical Co.Ltd |
| Abstract: | Objective：To establish a HPLC method for determination of the content of potassium dehydroandrograpolide succinate injection.Methods：Diamonsil C18high performance liquid chromatography column （250mm×4.6mm,5μm） produced by Dikma technologies was used.The mobile phase consisted of acetonitrile -water （35 -65）with phosphoric acid） -methanol（6：13）,and the flow rate was 1.0ml/min.EvaporativeLight -scattering Detector.Results：Astragaloside Ⅳ and it′s related substances couldbe separated well.The linearity of the dehydroandrographolide Succinate curve was well correlated（r=0.999698）within the range of 0.03021mg/ml -0.19206mg/ml.The instrument precision RSD was 2.81%;The method precision RSD was 3.78%.Conclusion：The methods appeared to be simple,accurate and reproducible.It can be used to control the quality of potassium dehydroandrograpolide succinate injection. |
| Keywords: | HPLC Astragaloside Ⅳ assay |
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