PCNA及bcl-2基因在膀胱移行细胞癌组织中的表达及其意义

目的 :探讨PCNA和bcl 2基因在膀胱癌组织中的表达及其预后意义 ,了解肿瘤增殖与凋亡的特点 ,揭示细胞凋亡和增殖在膀胱癌发生发展中的作用。方法 :采用免疫组织化学LSAB法 ,对 78例膀胱移行细胞癌组织进行PCNA和bcl 2表达的检测。结果 :在膀胱癌中 ,PCNA主要呈胞核表达 ,bcl 2呈胞质表达 ,PCNA和bcl 2染色阳性率分别为 35 9%和 5 6 4 % ;PCNA表达与膀胱移行细胞癌组织分级、预后呈正相关 (P =0 0 1,P =0 0 386 ) ,bcl 2亦与肿瘤分级、预后呈高度正相关 (P =0 0 0 0 2 ,P =0 0 116 ) ;PCNA和bcl 2表达无明显相关 (P =0 2 32 7)。结论 :PCNA和bcl 2参与膀胱癌的恶性转化、侵袭及进展的过程 ;检测PCNA和bcl 2有助于判断膀胱肿瘤恶性分化程度 ,并且二者可以作为预后的评价指标

Expression and Implication of PCNA and Bcl-2 in Bladder Transitional Cell Carcinoma

Objective To study PCNA and bcl-2 expressions in bladder transitional cell carcinoma and to evaluate their prognostic significance in order to explore the relationship between the immunohistochemical stainings and biological behavior of bladder cancer.Methods PCNA and bcl-2 expressions were studied by immunohistochemical methods(LSAB methods) in 78 samples of bladder transitional cell carcinomas(TCC) and their clinical significance was analysed. Results The positive rates of PCNA and bcl-2 were 35.90% and 56.41% respectively,PCNA was positively related to the tumor grade and the prognosis of the patient(P=0.01, P=0.038 6),and bcl-2 was highly positively related to the grade and prognosis too(P=0.000 2,P=0.011 6).On the other hand,no significant correlation has been noted between PCNA and bcl-2 (P=0.232 7).Conclusions The detection of the expressions of PCNA and bcl-2 is in favor of estimating the degree of differentiation and they might serve as useful prognostic markers for bladder cancer.