芦笋多糖提取、单糖组分分析及定量测定

目的提取芦笋多糖,纯化后分析其单糖组分并测定总糖,为今后芦笋多糖研究提供参考。方法水提醇沉法提取粗多糖,Sevage法除蛋白,H2O2脱色,高效毛细管电泳(HPCE)测定单糖组成,苯酚-硫酸法测定多糖。结果芦笋粗多糖主要单糖组成为木糖、果糖、鼠李糖、阿拉伯糖、半乳糖,其质量分数分别为3.44%、7.92%、10.52%、17.15%、41.85%。以单糖混合物为对照品测得粗多糖中总糖的质量分数为79.14%。结论高效毛细管电泳分离度高,可用于单糖组分分析;苯酚-硫酸法实验操作简便,准确可靠,可用于芦笋多糖的测定。

Monosaccharide composition analysis and its content determination in polysaccharide extract from Asparagus officinalis

Objective To analyze the monosaccharide composition in the polysaccharide extract from Asparagus officinalis, determine the content of monosaccharide, and provide some references for further research. Methods The monosaccharide composition was determined by HPCE. Phenol-sulfuric method was used for the determination of the content of monofsaccharide. Results The monosaccharide composition in polysaccharides from A. officinalis is Xyl, Fru, Rha, Ara, and Gal, their contents were 3.44%, 7.92%, 10.52%, 17.15%, and 41.85%, respectively. The content of polysaccharide is 79.14%, taking the mixture of monosaccharide compositions as reference substances. Conclusion HPCE can be used to analyze the monosaccharide composition in the polysaccharides with high precision of separation and the method of phenol-sulfuric acid is simple, convenient, and reliable.