高效毛细管电泳二极管阵列检测法同时测定地黄饮片中5种指标成分的含量

 目的 建立高效毛细管电泳二极管阵列检测法（HPCE-DAD）同时测定地黄饮片中梓醇、桃叶珊瑚苷、栀子苷、毛蕊花糖苷和肉桂酸含量的方法。方法 以60 mmol·L¹硼砂缓冲液(5%甲醇，pH 9.5) 为电泳介质，未涂渍标准熔融石英毛细管(75 μm×64.5 cm， 有效长度56 cm)为分离通道，分离电压为20 kV，检测波长为210 nm，毛细管温度为25 ℃，压力进样为5 kPa×6 s。结果 5种指标成分的浓度与峰面积的线性关系良好( r > 0.997 8) ；加样回收率为97.63%～103.03%。用此法测定了地黄饮片中5种指标成分的含量，得到满意结果。结论 该方法简单、准确，重复性较好，可用于地黄饮片内在质量的评价和控制。

Simultaneous Determination of Five Index Components in Rehmanniae Radix by HPCE-DAD

OBJECTIVE To establish a high performance capillary electrophoresis method with diode array detection (HPCE-DAD) method for simultaneous determination of catalpol， aucubin， geniposide，acteoside and cinnamic acid in raw and processed Rehmanniae Radix. METHODS 60 mmol·L^-1sodium borate was used as buffer solution (5% methanol， pH 9.5)， uncoated fused silica capillary (64.5 cm×75 μm， 56 cm of effective length ) was used， separation voltage was 20 kV， detection wavelength was 210 nm， column temperature was maintained at 25 ℃， and sample was injected at 5 kPa×6 s. RESULTS Five index components showed good linearity ( r >0.997 8) in the ranges of the tested concentrations， the average recoveries of the method were between 97.63%-103.03%. CONCLUSION The method is simple， accurate and reproducible， and can be used for the quality control of Rehmanniae Radix.