载脂蛋白E和脂蛋白脂酶基因多态性与冠心病关系的研究

目的:研究载脂蛋白E(apoE)基因和脂蛋白脂酶(LPL)基因多态性与冠心病的关系。方法:选择80例冠心病患者(CHD组),对照组为60例健康人,通过聚合酶链反应-限制性片段长度多态性测定apoE基因和LPL基因多态性。结果:CHD组携带E3/4基因型较对照组增高,对照组携带E3/3较CHD组增高(P<0.05)。E4等位基因频率分布CHD组明显高于对照组(P<0.05);LPL基因型分布和等位基因频率在CHD组和对照组间差异无统计学意义(P>0.05)。apoE和LPL基因多态性与冠脉病变严重程度无相关性。apoE和LPL两基因联合比较发现,携带E4等位基因与否的不同LPL基因型在CHD组和对照组间无统计学差异(P>0.05)。结论:apoEE4等位基因可能是冠心病的遗传易感因子;LPL基因型与冠心病发病无显著关联,apoE和LPL基因型在冠心病发病中无明显相互作用。

Study on correlation between apoliprotein E gene and lipoprotein lipase gene polymorphisms with coronary heart disease

Objective To study the relation of apoliprotein E (apoE) and lipoprotein lipase(LPL) gene polymorphisms with CHD. Methods 80 people with CHD were chosen as case group , 60 normal people were chosen as the control group. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the apoE gene and LPL gene polymorphisms. Results The requencies of 3/4 genotypes in CHD group were significantly higher than in control group . The frequencies of E3/3 were lower in CHD group than in control group. A significant difference was found in E4 allele between two groups （P＜0.05）. There were no significantly difference in LPL genotype and alleles frequencies between two groups(P＞0.05). apoE gene and LPL gene polymorphisms were found not related to the severity of CHD.There were no differences in various LPL genotypes when carrying E4 allele between two group either (P＞0.05). Conclusion apoE E4 allele is probably the genetic marker of CHD . LPL gene polymorphism has no relation with CHD .No interaction effect with apoE gene and LPL gene at the occurrence of the CHD.