8—氯腺苷对生长因子诱导的视网膜色素上皮细胞增殖抑制作用的研究

目的探讨８氯腺苷（８ｃｈｌｏｒｏａｄｅｎｏｓｉｎｅ,８ＣＬＡ）对生长因子诱导的视网膜色素上皮（ｒｅｔｉｎａｌｐｉｇｍｅｎｔｅｐｉｔｈｅｌｉｕｍ,ＲＰＥ）细胞增殖的抑制作用。方法通过ＲＰＥ细胞培养,采用３ＨＴｄＲ掺入测定８ＣＬＡ对肿瘤坏死因子α（ｔｕｍｏｒｎｅｃｒｏｓｉｓｆａｃｔｏｒ,ＴＮＦα）、白细胞介素１β（ｉｎｔｅｒｌｅｕｋｉｎ,ＩＬ１β）和碱性成纤维细胞生长因子（ｂａｓｉｃｆｉｂｒｏｂｌａｓｔｇｒｏｗｔｈｆａｃｔｏｒ,ｂＦＧＦ）诱导的ＲＰＥ细胞ＤＮＡ合成的抑制作用。结果三种因子单独使用均可使ＲＰＥ细胞３ＨＴｄＲ掺入每分钟计数（ｃｏｕｎｔｐｅｒｍｉｎｕｔｅ,ＣＰＭ）值明显增高（Ｐ＜０．０５）,８ＣＬＡ在其终浓度为２～１６μｍｏｌ／Ｌ时可使三种因子刺激的ＲＰＥ细胞３ＨＴｄＲＣＰＭ值明显降低（Ｐ＜０．０５）。在ＴＮＦα、ＩＬ１β、ｂＦＧＦ和８ＣＬＡ组,分别于１６、８、２μｍｏｌ／Ｌ时,ＣＰＭ值与基础值相近（Ｐ＞０．０５）。结论８ＣＬＡ可抑制生长因子诱导的ＲＰＥ细胞增殖,为抗增殖药物提供了新的途径

8-chloroadenosine inhibits the growth factor-induced proliferation of retinal pigment epithelial cells in vitro

OBJECTIVE: To study the inhibitory effect of 8-chloroadenosine (8-Cl-A) on growth factor-induced proliferation of retinal pigment epithelial (RPE) cells in vitro. METHODS: Primary culture and subculture of bovine RPE cells were established in vitro first and then tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), basic fibroblast growth factor (bFGF) plus different concentrations of 8-Cl-A (2-16 micromol/L) were added to the cells of third passage respectively. DNA synthesis in RPE cells was measured with (3)H-TdR incorporation. RESULTS: Cells treated with TNF-alpha, IL-1beta, bFGF had a higher (3)H-TdR uptake than the basal. The increase was abolished by 8-Cl-A in a dose dependent manner (P < 0.05). At TNF-alpha, IL-1beta, bFGF with 8-Cl-A group, CPM was similar to basal at 16, 8, 2 micromol/L respectively (P > 0.05). CONCLUSION: 8-Cl-A can inhibit the three growth factor-induced proliferation of RPE cells.