| RNA干扰抑制人肝癌细胞N-ras基因表达的研究 |
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| 引用本文： | 赵岳,徐凯成,张燕菊,李中辉,刘伟.RNA干扰抑制人肝癌细胞N-ras基因表达的研究[J].中国实验诊断学,2010,14(2):177-179. |
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| 作者姓名： | 赵岳 徐凯成 张燕菊 李中辉 刘伟 |
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| 作者单位： | 1. 吉林大学中日联谊医院,吉林,长春130033
2. 汪清林业局职工医院,吉林,汪清133200 |
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| 摘 要： | 目的研究RNA干扰(RNAi)对BEL7402细胞的N-ras基因表达的干扰效应。方法建立装载靶向N-ras基因小干扰性RNA(siRNA)的表达载体;用脂质体法将此表达载体转染BEL7402细胞株,以转染空载细胞作为对照;采用RT-PCR和Western blot检测N-ras基因的表达;流式细胞仪检测细胞凋亡情况。结果转染siRNA的表达载体可以抑制内源N-ras基因在转录和转译上的表达。N-ras基因的表达抑制与BEL7402细胞的凋亡相关联。结论转染siR-NA的表达载体可明显抑制肝癌细胞株BEL7402N-ras基因的表达,并伴有细胞的凋亡。其工作的开展将为RNAi治疗、肝癌N-ras基因功能研究、新药开发提供研究基础。
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| 关 键 词： | RNA干涉 肝癌 N-ras基因 |
Suppression of N-ras expression by interference RNA in BEL7402 hepatocellular carcinoma cells |
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| ZHAO Yue,XU Kai-cheng,ZHANG Yan-ju,et al..Suppression of N-ras expression by interference RNA in BEL7402 hepatocellular carcinoma cells[J].Chinese Journal of Laboratory Diagnosis,2010,14(2):177-179. |
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| Authors: | ZHAO Yue XU Kai-cheng ZHANG Yan-ju |
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| Institution: | ZHAO Yue,XU Kai-cheng,ZHANG Yan-ju,et al.(China-Japan Union Hospitol,Jilin Univercity,Changchun 130033,China) |
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| Abstract: | Objective To study the inhibitory effect of RNA interference (RNAi) on N-ras expression in hepatocellular carcinoma cell line,BEL7402.Methods Expression vector of N-ras gene targeting small interference RNA (siRNA) was constructed (psilencer-1-N-ras) and transfected into BEL7402.cells by lipofectamine,and the unloaded vector was used as control (mock).The expression of N-ras mRNA and protein was identified by RT-PCR and Western blot.Apoptosis of the transfected cells was examined by flow cytometry.Results A... |
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| Keywords: | Interference RNA Hepatocellular carcinoma N-ras gene |
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