两种快速检测耐甲氧西林金黄色葡萄球菌下呼吸道感染标本的方法评估

目的评价两种快速检测耐甲氧西林金黄色葡萄球菌(MRSA)下呼吸道感染标本的临床应用价值。方法 167份下呼吸道感染标本同时用直接多重PCR检测法、免疫富集联合多重PCR检测,并以常规细菌培养+PBP2a胶乳凝聚法检测结果为标准,评价两种方法的敏感性、特异性。结果直接多重PCR法检出MRSA4株,总检测率为2.3%;免疫富集联合多重PCR法检出MRSA20株,总检测率为11.9%;细菌培养+PBP2a胶乳凝集法为对照,免疫富集联合多重PCR检测法、直接采用多重PCR检测法的敏感性分别为100.0%、28.6%,特异性分别为96.1%、100.0%。结论免疫富集联合多重PCR法灵敏度高、特异性强、简单快速,适用于直接快速检测下呼吸道感染标本中的MRSA,该方法对及早发现、诊断MRSA感染患者有重要的意义。

Two Techniques for Rapid Detection of Meticillin-resistant Staphylococcus aureus Directly from Lower Respiratory Tract Infection Clinical Samples and Their Evaluation

OBJECTIVE To evaluate the clinical application of the two methods for detection of meticillin-reaistant Staphylococcus aureus (MRSA) directly from lower respiratory tract infection clinical samples.METHODS Directly Multiplex PCR and Multiplex PCR combined with immunomagnetic assay for simultaneously detecting 167 lower respiratory tract infection clinical samples were compared with culture-based MRSA screening latex agglutination test.RESULTS Four strains and twenty MRSA strains were detected by directly Multiplex PCR and Multiplex PCR combined with immunomagnetic assay respectively,and compared with culture-based MRSA screening latex agglutination,their sensitivity was 28.6%and 100%,whereas their specificity was 100%and 96.1%,respectively.CONCLUSIONS This Multiplex PCR combined with immunomagnetic assay is simple,rapid,sensitive and specific,and available in detecting lower respiratory tract infection clinical samples that contain a corresponding amount of DNA of MRSA.