白藜芦醇对小鼠Lewis肺癌细胞凋亡的影响

目的探讨白藜芦醇（Res）对小鼠Lewis肺癌细胞凋亡的影响。方法建立C^57BL小鼠Lewis肺癌模型，将40只接种Lewis肺癌的C^57BL小鼠随机分成4组：对照组、Res低剂量组（2．5mg／kg·d）、Res中剂量组（5mg／kg·d）和Res高剂量组（10mg／kg·d），每组10只。连续灌胃20日，于接种后第22天处死小鼠，检测肿瘤体积及重量，用原位末端标记法（TUNEL）检测肿瘤细胞凋亡指数（AI）。结果Res中、高剂量组肿瘤的生长明显受到抑制，瘤重和肿瘤体积明显低于对照组（P〈0．01），其抑瘤率分别为39．41％、50．35％，明显高于Res低剂量组（11．81％），差异均有显著性（P〈0．01）。Res中、高剂量组AI明显高于对照组（P〈0．01），但低剂量Res对AI无明显影响。结论白藜芦醇可明显抑制小鼠Lewis肺癌的生长，其机制可能与促进细胞凋亡有关。

Influence of Resveratrol on Cell Apoptosis of Lewis Lung Carcinomas

Objective To explore the influence of resveratrol （Res） on apoptosis Of Lewis lung carcinoma in mice. Methods The Lewis lung carcinoma models of C^57BL mice were established. Forty C^57BL mice were randomly divided into 4 groups： control group, low - dose Res group （ 2.5 mg/kg · d ）, middle - dose Res group （5 mg/kg · d ） and high - dose Res group （ 10mg/kg · d ）. There are 10 mice in every group. Different treatments were served from day 2 after transplantation and all mice were sacrificed after 22 days. The volume and weight of tumors were detected. Apoptosis index （ AI ） was determined by TUNEL assay. Results The tumor growth was suppressed significantly in middle - dose and high -dose Res group. The weight and volume of tumor were markedly decreased in those groups compared with control group （ P 〈 0.01 ） and their inhibitory rates were 39.41% and 50.35% respectively, which was higher than low - dose Res group（ 11.81% ） （P 〈 0.01）. AI was enhanced in middle- dose and high- dose group compared with control group （P 〈 0.01 ）. However,low - dose Res had no obvious influence on AI （ P 〉 0.05 ）. Conclusion Resveratrol can remarkably inhibit Lewis lung carcinoma growth in mice. Its mechanism might be associated with promoting apoptosis.