Isolation of ovine rotavirus in cell cultures |
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| Authors: | T. Makabe H. Komaniwa Y. Kishi K. Yataya H. Imagawa K. Sato Y. Inaba |
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| Affiliation: | (1) Tochigi Livestock Sanitary Laboratory, Utsunomiya, Tochigi, Japan;(2) Epizootic Research Station, Equine Research Institute, Japan Racing Association, Kokubunji, Tochigi, Japan;(3) National Institute of Animal Health, Tsukuba, Ibaraki, Japan |
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| Abstract: | Summary Three cytopathic rotavirus strains were isolated in MA104 cells from intestinal contents of lambs with diarrhea. Pretreatment of virus with pancreatin and incorporation of a small amount of pancreatin in maintenance medium were important for establishment of the strains in these cells. The isolates showed marked cross reactions with bovine rotavirus in immunofluorescence, but no cross reaction with bovine, human, simian, equine, porcine and lapine rotaviruses in neutralization. |
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