螺杆菌感染与原发性肝癌的相关性研究

目的　探讨螺杆菌感染与人类原发性肝癌(PLC)的相关性。方法　选取 21例PLC患者的肝癌组织为研究对象(实验组), 12例非肝癌手术患者的肝组织作对照组。参照文献设计螺杆菌属细菌 16SrRNA基因特异性引物,进行聚合酶链反应 (PCR)扩增,扩增产物经Southern杂交证实,并将PCR产物进行测序及同源性比较。同时,将两组标本制成病理切片用于螺杆菌属的cDNA mRNA原位杂交实验。通过定性和定位测定评价肝组织螺杆菌感染与PLC的相关性。结果　实验组 62%(13 /21)的肝癌组织中检出螺杆菌属 16SrRNA存在,而对照组无阳性检出 (P<0 .01)。所有PCR产物经Southern杂交得到证实。利用原位杂交技术检测实验组和对照组标本螺杆菌属 16SrRNA mRNA,实验组有 13例阳性,对照组均为阴性 (P<. 0 01 )。比较显示,肝癌组织中的螺杆菌属16SrRNA序列与幽门螺杆菌的 16SrRNA序列有 97 80%的同源性。结论　PLC患者肝组织中可能存在螺杆菌属感染且感染率较高,提示螺杆菌感染与PLC的发生可能存在某种相关性。

Association between Helicobacter infection in liver tissue and primary liver carcinoma

Objective To investigate the relationship between Helicobacter species and primary liver carcinoma (PLC). Methods The liver samples resected during operation from 21 patients with PLC diagnosed by histopathology and 12 patients with other liver diseases as controls were studied. Helicobacter species in liver specimens from the studied subjects were examined by PCR with Helicobacter specific 16SrRNA primers. The amplified products were identified by Southern hybridization and nucleic acid hybridization in situ and sequencing. The specimens were made slices to undergo in situ hybridization of cDNA mRNA of Helicobacter . Qualitative and quantitative studies were used to assess the correlation of liver tissue helicobacter infection with PLC. Results Thirteen of the 21 samples (62%) of PLC were positive for Helicobacter specific 16SrRNA gene, while none was positive in the controls ( P <0 01). In situ hybridization results demonstrated a highly Helicobacter 16SrRNA mRNA positive rate in the PLC group (62%) and none of positive specimen in the control group ( P <0 01).Nine of the Helicobacter specific PCR applicants were sequenced and a homology of 97 80% in comparison with 16SrRNA of H p was found. Conclusion Helicobacter infection may exist in the liver tissues of PLC patients with a high infection rate, suggesting an association between Helicobacter infection and PLC.