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| 食品中肠出血性大肠埃希菌O157:H7的PCR检测 | |
| 引用本文： | 邢立新,孙武长,刘桂华,乔凤,龚云伟,黄鑫.食品中肠出血性大肠埃希菌O157:H7的PCR检测[J].中国公共卫生,2006,22(12):1504-1505. |
| 作者姓名： | 邢立新 孙武长 刘桂华 乔凤 龚云伟 黄鑫 |
| 作者单位： | 1. 吉林省卫生厅卫生监督所,长春,130062 2. 吉林省疾病预防控制中心 3. 吉林大学 4. 吉林省长春市疾病预防控制中心 |
| 摘 要： | 目的建立快速准确检测食品中肠出血性大肠埃希菌O157：H7的方法。方法采用多重聚合酶链式反应技术（MPCR）特异性扩增肠出血性大肠埃希菌O157：H7的STX、rfbE、fliC基因。结果分别在228，378，709bp处扩增出3个目的基因片段，且只有肠出血性大肠埃希菌O157：H7获得扩增，其他菌种扩增均呈阴性。结论PCR方法比传统细菌检测方法更特异、快速、灵敏和简便，为食品中肠出血性大肠埃希菌O157：H7的快速检测提供了新的手段。 |
| 关 键 词： | 食品 肠出血性大肠埃希菌O157:H7 聚合酶链式反应 |
| 文章编号： | 1001-0580（2006）12-1504-02 |
| 收稿时间： | 2006-04-10 |
| 修稿时间： | 2006-04-10 |
Detection of Enterohemorrhagic escherichia coli O157: H7 in food with PCR assay |
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| XING Lixin, SUN Wuchang, LIU Gui- hua,et al..Detection of Enterohemorrhagic escherichia coli O157: H7 in food with PCR assay[J].Chinese Journal of Public Health,2006,22(12):1504-1505. | |
| Authors: | XING Lixin SUN Wuchang LIU Gui- hua |
| Institution: | Jilin Provincial Institute for Health Inspection and Supervision ,Changchun 130062, china |
| Abstract: | Objective To achieve rapid detection of Enterohemorrhagic escherichia coli O_(157):H_7 in food was achieved by using multiplex PCR assay.Methods The virulence genes of EHEC :coli O_(157):H_7 like toxin STX,rfbE,flic were chosen as the terget genes and amplified in multiplex PCR assays.Results The assay was designed to amplify the 228,378 and 709 bp regions of cornesponding virulence three genes And onl EHEC :coli O_(157):H_7 was amplified.No specific amplication product was producied from other strains.Conclusion Conclusion PCR method is more concise,rapid,sensitive and more specific than conventional culture method.It is proved to be a new method for rapid detection EHEC :coli O_(157):H_7 in food. |
| Keywords: | food Enterohemorrhagic escherichia coli O_(157):H_7 PCR |
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