过氧化物酶体增殖活化受体γ基因Pro12Ala和C161T多态性与溃疡性结肠炎的相关性

目的研究过氧化物酶体增殖活化受体γ（PPAR-γ）基因Pro12Ala和C161T位点的多态性与湖北汉族人群溃疡性结肠炎（UC）的相关性。方法采用多聚酶链式反应-限制片段长度多态性分析方法（PCR-RFLP），对212例UC患者和220例健康对照者进行PPAR-γ基因分型。结果UC患者PPAR-γ基因C161T位点C／T基因型频率，T等位基因频率明显高于正常组（35．4％比25．0％，P=0．015，OR=1．69，95％CI=1．12～2．57；20．1％比12．9％，P=0．006，OR=1．68，95％CI=1．17～2．43），T等位基因携带者也高于正常对照组（37．7％比25．5％，P=0．007，OR=1．77，95％CI=1．18～2．68）。而Pro12Ala位点基因型频率，等位基因频率UC组与对照组比较差异无统计学意义（P〉0．05）。结论PPAR-γ基因C161T位点基因T等位基因与湖北汉族人群UC的发生存在相关性。

Correlation of peroxisome proliferator-activated receptor-gamma Pro12Ala and C161T polymorphisms with ulcerative colitis

Objective Peroxisome proliferator-aetivatod receptor-gamma （ PPAR-γ ） is frequently expressed in colon and polymorphisms in the PPAR-γ gene may play a role in the etiology of inflammatory bowel disease（IBD）. The aims of the present study were to determine the correlations of PPAR-γ Pro12Ala and C161T polymorphisms with ulcerative colitis （UC）. Methods Two hundred and twelve ulcerative colitis patients and 220 age and sex matched healthy controls were enrolled at Zbongnan Hospital of Wuhan University in China. The PPAR-γ Pro12Ala and C161T polymorphisms were genotyped by a method of polymerase chain reaction-restriction fragment length polymorphism. Results In C161T polymorphism, the prevalence of C/T genotype and T allele was higher in UC patients than in controls（ C/T genotype ：35.4% vs25.0% ,P=0.015,OR = 1.69,95% CI = 1.12 -2.57;T allele：20.1% vs12.9% ,P =0. 006,OR = 1.68,95% CI = 1.17 - 2.43）. When combining C/T and T/T genotypes, the frequency of T carriers was more common in UC patients than in controls（37.7% vs 25.5% ,P =0. 007 ,OR = 1.77,95% CI ： 1.18 -2.68）. The polymorphism of Pro12Ala genotype did not show significant association with UC patients. Conclusion Our study suggests the potential association between C161T polymorphism in the PPAR-γ gene and UC patients.