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Purification of bovine conglutinin using pepsin digestion |
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| Authors: | M A Maire M Barnet P H Lambert |
| Institution: | WHO Immunology Research and Training Centre, Centre de Transfusion, Hôpital Cantonal, 1211 Geneva 4, Switzerland |
| Abstract: | This paper describes a new method for the purification of bovine conglutinin based on the relative resistance of this protein to pepsin digestion. First, conglutinin is purified by absorption on yeast, then the preparation is treated with 2% pepsin (w/w) at 4°C for 18 hr, and finally gel filtrated on agarose A5m. The yield is 60–75% and conglutinin thus prepared appears physically, immunochemically and functionally intact. This procedure allows for a rapid production of sufficient amounts of conglutinin for immune complex detection or purification methods. |
| Keywords: | EDTA ethylenediaminetetraacetate SDS sodium dodecylsulfate K conglutinin C3bi inactivated third component of complement KGB conglutinin-binding test PMMA polymethylmetacrylate PBS phosphate buffered saline BIS TEMED Tris tris (hydroxymethyl)-aminomethane HMW high molecular weight DEAE diethylaminoethyl- |
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