Sequence of a new DR12 allele with two silent mutations that affect PCR-SSP typing |
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Authors: | Zanone R Bettens F Tiercy J-M |
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Affiliation: | National Reference Laboratory for Histocompatibility, Transplantation Immunology Unit, University Hospital, Geneva, Switzerland. |
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Abstract: | A new HLA-DR12 allele has been identified in a European Caucasoid bone marrow donor. The DRB1*12012 allele differs from DRB1*12011 by two silent substitutions at codons 72 and 78, two polymorphic positions used for DNA subtyping of the DR12 serotype. The co-occurence of the two nucleotide changes is unique to the DR12 group and results in a new PCR-SSP typing pattern. The complete HLA type of the donor is A24, A68; B55, B61; Cw*01, Cw*0304; DRB1*12012, DRB1*1402; DRB3*0101, DRB3*0202; DQB1*0301. HLA-DRB1*12012 is a rare allele as it occurs in < 0.2% of DR12 donors. |
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Keywords: | HLA-DR12 microtiter plate oligotyping PCR-SSP sequencing silent substitutions |
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