HPLC法测定双花百合片中绿原酸的含量

目的：建立双花百合片中绿原酸的含量测定方法。方法：采用HPLC法，色谱柱为Eelispse XDB C18 （4.6 mm×250 mm, 5 μm）色谱柱，流动相为0．4％磷酸溶液-乙腈（85：15），柱温为25℃，流速为0．8ml／min，检测波长为327nm。结果：绿原酸在0．10-5．00μg范围内呈良好的线性关系（r=O．9997），平均回收率为99．60％，RSD=1．56％（n=6）；测得3个批次双花百合片样品中绿原酸含量分别为0．08、0．10、0．09mg／片。结论：该方法操作简单，灵敏度高，重现性好，测定结果可靠，可用于双花百合片中绿原酸含量的测定。

Determination of chlorogenic acid in Shuanghua Baihe tablet by HPLC

Objective： To establish the method for determination of the chlorogenic acid in Shuanghua Baihe tablet. Methods： Eelispse XDB C18 （4.6 mm×250 mm, 5 μm） was used as stationary phase,. 0.4% phosphoric acid-aeetonitrile （15：85） was used as mobile phase, at the rate of 0.8 ml/min, the detective wavelength was 327 nm. Results： The linear range of chlorogenic acid was between 0.10 and 5.00 μg （r=-0.999 7）, and the average recoveries was 99.60%, RSD was 1.56% （n=6）. Each tablet contains chlorogenic acid 0.08, 0.10, 0.09 mg in 3 batches of sample. Conclusion： The method is simple, high sensitivity, good reproducibility and reliable in results, and can be used as the determination of the chlorogenic acid in Shuanghua baihe tablet.