| 培养液成分对胚胎体外发育的影响 |
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| 引用本文： | 成俊萍,石德顺,李日聪,陆凤花,韦精卫. 培养液成分对胚胎体外发育的影响[J]. 国外畜牧学(草食家畜), 2005, 0(4): 39-41 |
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| 作者姓名： | 成俊萍 石德顺 李日聪 陆凤花 韦精卫 |
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| 作者单位： | 广西大学动物繁殖研究所,广西,南宁,530004;广西大学动物繁殖研究所,广西,南宁,530004;广西大学动物繁殖研究所,广西,南宁,530004;广西大学动物繁殖研究所,广西,南宁,530004;广西大学动物繁殖研究所,广西,南宁,530004 |
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| 摘 要： | 牛体外受精、棱移植或转基因的早期胚胎需体外培养到桑椹胚或囊胚后才能非手术移植到受体母牛受孕。本文综述了体外培养液中能量培养基，血清、牛血清白蛋白（BSA）和聚乙烯醇（PVA），生长因子、胞浆因子和细胞外基质分子，pH值，EDTA、NaN3和2，4-二硝基苯酚等对胚胎体外培养的影响。
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| 关 键 词： | 胚胎 培养液 体外培养 |
| 文章编号： | 1003-6377（2005）04-0039-03 |
| 收稿时间： | 2005-08-18 |
| 修稿时间： | 2005-08-18 |
Effects of Culture Medium Ingredients on the in Vitro Development of Embryos |
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| CHENG Jun-ping,SHI De-shun,Li Ri-cong,LU Feng-hua,WEI Jing-wei. Effects of Culture Medium Ingredients on the in Vitro Development of Embryos[J]. Grass-Feeding Livestock, 2005, 0(4): 39-41 |
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| Authors: | CHENG Jun-ping SHI De-shun Li Ri-cong LU Feng-hua WEI Jing-wei |
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| Affiliation: | Animal Reproduction Institute, Guangxi Universty,Nanning 530004, China |
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| Abstract: | Zygotes and early embryos derived from nuclear transfer or gene targeting must be cultured in vitro to the morula and blastocyst stage at which can be transferred into recipients by unsurgery.In this paper,the influence of the ingredients of culture medium on the embryo developing in vitro were reviewed,such as serum bull serum albumin(BSA) or polyvinyl alcohol,growth factors,cytokines and extra-cellular matrix molecules,ethylenediamine tetraacetic acid(EDTA),NaN_3 and 2,4-dinitrophenol. |
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| Keywords: | Embryo Culture medium In vitro culture |
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