海南省麻疹野病毒的分离与血清学初步研究

目的：为了解海南省麻疹暴发病例中，现行麻疹野病毒流行状况，以及本土麻疹病毒的基因型，方法：采集麻疹疑似病例咽试子标本，应用B95a细胞进行病毒分离培养。结果：在5例疑似病例中分离到2株麻疹病毒，阳性率为40％，经RT－PCR和其产物的基因序列分析，属H1基因型，为中国本土麻疹野毒株，同时采用麻疹疫苗初免儿童血清中和新分离麻疹病毒，结果中和抗体滴度为1：10，1：20，说明我国生产的麻疹疫苗对我省现流行的麻疹野病毒能起到保护作用。结论：这是海南省首次用B95a细胞成功分离到麻疹野病毒，并通过基因分型证实为中国本土野病毒，填补了海南省麻疹病毒分离的空白。

Preliminary serological study and isolation of measles viruses in Hainan Province

ve To know the current wild type measles virus and it' s genotype that circulated in Hainan Province. Methods Swallow swab samples of suspected measles cases were collected and isolated virus with B95a cell line. Result Two measles virus strains were isolated from five samples. The positive rate was 40% .RT- PCR and it's product sequence analysis indicated that the two wild - type measles virus strains belong to H1 genotype. It was indigenous measles virus of China. Meanwhile serum immunized by measles vaccine was used to neutralize the two measles virus strains. The neutralization antibody titer was 1:10, 1:20. The result showed that the measles vaccine could protect children from current wild - type measles virus. Conclusion Measles virus was isolated successfully with G95a cell line in Hainan province and was the first identified indigenous wild type measles virus.