| Anti-angiogenesis Effect on Glioma of Attenuated Salmonella Typhimurium Vaccine Strain with flk-1 Gene |
| |
| 作者姓名： | 冯珂珂 赵洪洋 陈剑 姚东晓 姜小兵 周伟 |
| |
| 作者单位： | DepartmentofNeurosurgery,UnionHospital,TongjiMedicalCollege,HuazhongUniversityofScienceandtechnology,Wuhan430022,China |
| |
| 摘 要： | To investigate the anti-vasculature effects and the anti-glioma effects of attenuated Salmonella typhimurium vaccine strain expressing VEGFR2 (flk-1) gene, plasmid pcDNA3, 1-flk1 was constructed and electro-transfected into live attenuated Salmonella typhimurium strain SL7207. Mouse models of intracranial G1261 glioblastoma were treated with an orally administered attenuated Salmonella typhimurium expressing flk-1 gene. The survival period was recorded and vessel density was observed by immunofluorescence. CTLs activity was measured by MTT assay. Our results showed that attenuated Salmonella typhimurium vaccine strain expressing flk-1 gene could significantly inhibit glioblastoma growth, reduce vessel density, prolong the survival period and improve the survival rate in these mice. The flk-1 specific CTLs activity was increased obviously after the vaccination. Our study showed that attenuated Salmonella typhlmurium vaccine strain expressing flk-1 gene could break peripheral immune tolerance a in glioma gainst this self-antigen and kill endothelial cells by the orally administered vaccine and can be used for both prophylactic and therapeutic purposes.
|
| 关 键 词： | 血管生成 神经胶质瘤 肿瘤 减毒作用 沙门氏菌 疫苗接种 flk-1基因 免疫荧光 |
Anti-angiogenesis Effect on Glioma of Attenuated Salmonella Typhimurium Vaccine Strain with flk-1 Gene |
| |
| FENG Keke,ZHAO Hongyang,CHEN Jian,YAO Dongxiao,JIANG Xiaobing,ZHOU Wei.Anti-angiogenesis Effect on Glioma of Attenuated Salmonella Typhimurium Vaccine Strain with flk-1 Gene[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2004,24(4):389-391. |
| |
| Authors: | FENG Keke ZHAO Hongyang CHEN Jian YAO Dongxiao JIANG Xiaobing ZHOU Wei |
| |
| Institution: | Department of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and technology, Wuhan 430022, China |
| |
| Abstract: | To investigate the anti-vasculature effects and the anti-glioma effects of attenuated Salmonella typhimurium vaccine strain expressing VEGFR2 (flk-1) gene, plasmid pcDNA3.1-flk1 was constructed and electro-transfected into live attenuated Salmonella typhimurium strain SL7207. Mouse models of intracranial Gl261 glioblastoma were treated with an orally administered attenuated Salmonella typhimurium expressing flk-1 gene. The survival period was recorded and vessel density was observed by immunofluorescence. CTLs activity was measured by MTT assay. Our results showed that attenuated Salmonella typhimurium vaccine strain expressing flk-1 gene could significantly inhibit glioblastoma growth, reduce vessel density, prolong the survival period and improve the survival rate in these mice. The flk-1 specific CTLs activity was increased obviously after the vaccination. Our study showed that attenuated Salmonella typhimurium vaccine strain expressing flk-1 gene could break peripheral immune tolerance a in glioma gainst this self-antigen and kill endothelial cells by the orally administered vaccine and can be used for both prophylactic and therapeutic purposes. |
| |
| Keywords: | attenuated Salmonella typhimurium flk-1 glioma |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
