Studies on iodinated compounds. X. Isolation and purification of iodoglycyltyrosines] |
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Authors: | M Miyashita R Matsunaga Y Seyama S Yamashita |
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Institution: | Department of Clinical Chemistry, Hoshi University, Tokyo, Japan. |
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Abstract: | The methods for the isolation and purification of iodoglycyltyrosines glycyl-3-iodotyrosine (Gly-MIT) and glycyl-3,5-diiodotyrosine (Gly-DIT)] from a reaction mixture were examined by the use of high-performance liquid chromatography (HPLC). Glycyltyrosine (Gly-Tyr) was iodinated with iodine monochloride (ICl) (the molar ratio of Gly-Tyr to ICl was 1:1.5) in 0.1 M NaOH. The synthesized Gly-MIT and Gly-DIT were separated on a mu Bondapak C18 column employing stepwise gradient systems of a 0.1% trifluoroacetic acid/acetonitrile mixture and a water/acetonitrile mixture. Chemically pure Gly-MIT and Gly-DIT were obtained in 30.2% and 28.2% yields, respectively. |
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