ATP-生物荧光法在筛选卵巢癌化疗药物中的应用

为了探讨ATP-生物荧光法在临床体外化疗药敏试验中应用的可行性，本文以卵巢癌细胞株A2780为实验模型，建立稳定和适宜的实验条件，并对卵巢癌临床化疗药物体外筛选进行了初步研究。结果表明该方法稳定性好，能检测出不同药物浓度对细胞作用的剂量-效应关系，最小检出细胞数为40个，实验变异系数小于8．2％(0．2％--8．2％)，预测准确率高(90．6％)。ATP-生物荧光法稳定性、重复性和敏感性均较好，可为临床化疗提供参考依据。

Application of ATP-bioluminescence Assay for Screening Chemotherapeutic Agents of Ovarian Cancer Chemotherapy

To evaluate the feasibility of using ATP-bioluminescence assay for tumor chemosensitivity testing in vitro, we selected the A2780 cell line as a model and established the suitable assay condition. Screening chemotherapeutic agents of ovarian cancer in vitro were preliminarily researched. Using this assay, dose-response curve was detected in cell line treated with these agents. The result showed that the coefficients of variation for assay ranged from 0.2% to 8.2%, which means high reproducibility.It can measured ATP content of as few as forty cells. The thermal stability of the luciferin-luciferase system was high enough used in industry. The predictable accuracy rate is about 90.6%. This study demonstrated ATP-bioluminescence assay is a reliable, reproducible and sensitive method.It can provide a technical base for screening sensitive chemotherapeutic agents in clinic.