茶多酚抑制人血管内皮细胞分泌单核细胞趋化蛋白1的研究

目的通过对人血管内皮细胞炎性分子单核细胞趋化蛋白1(MCP-1)的检测,评价茶多酚在血脂异常中对血管内皮的保护作用及其作用机制。方法应用人血管内皮细胞为研究模型,在基础或者氧化型低密度脂蛋白刺激的条件下,给予不同剂量的茶多酚,并与其他抗氧化剂水溶性维生素E(Trolox)、银杏提取物(EGb761)相比较,检测不同时间点细胞培养基当中的MCP-1含量。结果 24 h内浓度小于200μmol/L的茶多酚对内皮细胞没有明显的毒性作用,并且可以剂量依赖性地抑制内皮MCP-1的分泌(均为P<0.05)。与未处理组及其他抗氧化剂组相比,茶多酚处理组显著地降低了MCP-1的基因表达和蛋白分泌水平,并呈剂量依赖性,表现出较好的内皮保护及抗炎作用。结论与其他抗氧化剂相比,茶多酚可以在脂质氧化的条件下更好地通过抑制炎性因子的表达和分泌来保护内皮细胞。

Effects of Green tea polyphenols on monocyte chemoattractant protein-1 secretion in human vascular endothelial cells

Objective To evaluate the protective effect of green tea polyphenols(GTP) on human vascular endothelial cells and its mechanism.Methods Human vascular endothelial cells were used as study model.Under baseline and ox-LDL stimulated condition,the cells were pretreated with different concentration of GTP and other antioxidants including Trolox and EGb761,and then monocyte chemoattractant protein-1(MCP-1) in cell culture medium was detected.Results GTP had no apparent toxic effects on endothelial cells within 24 h when the concentration less than 200 μmol/L.Compared to untreated cells and other antioxidants treated cells,the expression of MCP-1 in GTP treated cells in both gene and protein level was significantly reduced in a dose-dependent manner(P<0.05).Conclusions GTP has protective effect on human endothelial cells by inhibiting the expression and secretion of MCP-1.