硝喹对体外培养的约氏疟原虫红内期膜磷脂的影响

目的:探讨硝喹抗疟作用机理。方法:用蜡烛缸法体外培养约氏疟原虫红内期,以3H]-乙醇胺掺入疟原虫膜磷脂作指标,观察硝喹对疟原虫膜磷脂合成的影响;以DPH作为荧光探针, 测膜荧光偏振度及微粘度。结果:硝喹明显抑制3H]-乙醇胺掺入约氏疟原虫,并增高约氏疟原虫膜荧光偏振度和微粘度。结论:硝喹可明显抑制疟原虫膜磷脂的生物合成,并明显降低膜流动性

EFFECT OF NITROQUINE ON THE MEMBRANE PHOSPHOLIPID OF INTRAERYTHROCYTIC PLASMODIUM YOELII IN VITRO

AIM: To study the mechanism of antimalarial action of nitroquine.METHODS:Intraerythrocytic P. yoelii was cultured by the method of Trager and Jensen. The amount of 3H] ethanolamine incorporation was measured as an index of the phospholipid synthesis.DPH was used as a probe to measure the plasmodial fluorescent polarization.RESULTS:The incorporation of 3H] ethanolamine into the P.yoelii infected erthrocytes was markedly inhibited by nitroquine. The plasmodial membrane polarization and viscosity were significantly increased by nitroquine.CONCLUSION: Nitroquine could inhibit the phospholipid synthesis and decrease the membrane fluidity of P.yoelii .