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脑型疟患者红细胞膜蛋白分子的表达
引用本文:边中启,王功焯,田学明,范江.脑型疟患者红细胞膜蛋白分子的表达[J].中国寄生虫学与寄生虫病杂志,1999,17(6):359-362.
作者姓名:边中启  王功焯  田学明  范江
作者单位:昆明总医院消化内科!昆明650032
摘    要:目的: 为研究脑型疟发病机制及防治提供理论依据。方法: 应用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳 (SDS-PAGE) 技术, 对云南省19 例脑型疟患者感染红细胞 (PE) 表达的红细胞膜蛋白1(PfEMP-1) 进行分析, 并分别与43 例恶性疟、9 例间日疟患者PE表达的PfEMP-1和PvEMP-1及6 例健康人红细胞膜蛋白(EMP) 进行比较。结果: 脑型疟患者PE存在高表达的高分子量PfEMP-1, 分子量为260~320 kDa。恶性疟及间日疟患者PE不表达260 kDa 以上的高分子量PfEMP-1,分别测到分子量最大为240kDa 的PfEMP-1和180 kDa的PvEMP-1。健康人对照组EMP分子量为140 kDa。结论: 脑型疟患者PE高表达的不同高分子量PfEMP-1 260~320 kDa, 与脑血管内皮细胞(EC) 不同受体蛋白CD36、血小板反应蛋白 (TSP)、细胞间粘附分子1 (ICAM-1)、血管细胞粘附分子1 (VCAM-1) 和内皮白细胞粘附分子1(ELAM-1) 及硫酸软骨素A (CSA) 的结合是脑型疟发病的分子基础, 可导致脑型疟患者发生昏迷。

关 键 词:脑型疟患者  恶性疟原虫  感染红细胞  表达  红细胞膜蛋白

EXPRESSION OF PLASMODIUM FALCIPARUM-INFECTED ERYTHROCYTE MEMBRANE PROTEIN FROM CEREBRAL MALARIA PATIENTS
BIAN Zhongqi,WANG Gongzhuo,TIAN Xueming,FAN Jiang.EXPRESSION OF PLASMODIUM FALCIPARUM-INFECTED ERYTHROCYTE MEMBRANE PROTEIN FROM CEREBRAL MALARIA PATIENTS[J].Chinese Journal of Parasitology and Parasitic Diseases,1999,17(6):359-362.
Authors:BIAN Zhongqi  WANG Gongzhuo  TIAN Xueming  FAN Jiang
Institution:Department of Gastroenterology, Kunming General Hospital, Kunming 650032.
Abstract:AIM: To provide theoretical cvidence for studying the molecular pathogenesis of human cerebral malaria.METHODS: The expressions of Plasmodium falciparum erythrocyte membrane protein 1(PfEMP1) on the surface of parasitized erythrocyte (PE) specimens from 19 cases of cerebral malaria patients in Yunnan Province were quantitatively analyzed by preparative sodium dodecyl sulfate\|polyacrylamide gel electrophoresis (SDS\|PAGE) technique. 43 patients of falciparum malaria, 9 patients of vivax malaria and 6 healthy controls were also investigated.RESULTS: The expressions of higher molecular mass (Mr) 260-320 kDa forms of PfEMP1 were found on PE from cerebral malaria patients. By contrast, the expression of PfEMP1 and P.vivax erythrocyte membrane protein (PvEMP1) on PE from falciparum malaria patients and vivax malaria patients had a PfEMP1 with Mr 240 kDa and a PvEMP1 with Mr 180 kDa band, respectively. Healthy controls expressed an EMP of Mr 140 kDa. CONCLUSION: The binding of 260-320 kDa PfEMP1 proteins expressed on PE from cerebral malaria patients to diverse receptor molecules on the endothelial cell(EC)of the cerebral microvessels such as CD36, thrombospondin (TSP), intercellular adhesion molecule 1(ICAM\|1), vascular cell adhesion molecule 1(VCAM\|1), endothelial leukocyte adhesion molecule 1(ELAM\|1) and chondroitin sulfate A (CSA) might be the molecular basis for the pathogenesis of cerebral malaria.
Keywords:Cerebral malaria patient  Plasmodium falciparum  parasitized erythrocyte  expression  erythrocyte membrane protein
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